Regulation of aromatase gene expression in the adult rat brain

Charles Roselli, Salah E. Abdelgadir, John A. Resko

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Brain aromatase plays an important role in the regulation of adult reproductive behavior in male rodents. This report focuses on recent experiments from our laboratory that examined the distribution and regulation of aromatase mRNA in the rat brain. Aromatase mRNA was measured by a highly sensitive ribonuclease protection assay using a 32P-labeled antisense RNA probe that was complimentary to the 5' coding region of rat aromatase mRNA. This probe protects two RNA fragments in rat brain tissue: a 430-nt length fragment and a shorter 300-nt fragment. The presence of the 300-nt RNA fragment is not associated with enzyme activity in the rat brain and appears to represent an alternative brain-specific aromatase transcript whose function, if any, is unknown. In contrast, the 430-nt RNA fragment represents mRNA, which is thought to encode functional aromatase enzyme because its levels are correlated with aromatase activity concentrations in preoptic area, hypothalamus, amygdala, and ovary. Aromatase activity and mRNA levels in the preoptic area and hypothalamus decreased by 7 days after castration and were maintained at intact levels by treatment with testosterone and dihyhdrotestosterone, but not with estradiol. In contrast, neither aromatase activity nor mRNA levels in the amygdala are affected by castration or hormone replacement. In addition, sex differences in the regulation of aromatase mRNA were apparent in both the preoptic area and hypothalamus. These results demonstrate that androgens regulate the transcription or stability of aromatase mRNA in specific brain areas. Moreover, they suggest that gender differences in androgen responsiveness play an important role in regulating gene expression in the adult rat brain.

Original languageEnglish (US)
Pages (from-to)351-357
Number of pages7
JournalBrain Research Bulletin
Volume44
Issue number4
DOIs
StatePublished - 1997

Fingerprint

Aromatase
Gene Expression Regulation
Brain
Messenger RNA
Preoptic Area
Hypothalamus
Castration
RNA
Amygdala
Androgens
RNA Probes
Reproductive Behavior
Antisense RNA
RNA Stability
Enzymes
Ribonucleases
Sex Characteristics
Testosterone
Ovary
Estradiol

Keywords

  • Amygdala
  • Aromatase
  • MRNA
  • Preoptic area
  • Rat brain
  • Sex differences

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regulation of aromatase gene expression in the adult rat brain. / Roselli, Charles; Abdelgadir, Salah E.; Resko, John A.

In: Brain Research Bulletin, Vol. 44, No. 4, 1997, p. 351-357.

Research output: Contribution to journalArticle

Roselli, Charles ; Abdelgadir, Salah E. ; Resko, John A. / Regulation of aromatase gene expression in the adult rat brain. In: Brain Research Bulletin. 1997 ; Vol. 44, No. 4. pp. 351-357.
@article{c9f922c6993944d6b73697de38558f7b,
title = "Regulation of aromatase gene expression in the adult rat brain",
abstract = "Brain aromatase plays an important role in the regulation of adult reproductive behavior in male rodents. This report focuses on recent experiments from our laboratory that examined the distribution and regulation of aromatase mRNA in the rat brain. Aromatase mRNA was measured by a highly sensitive ribonuclease protection assay using a 32P-labeled antisense RNA probe that was complimentary to the 5' coding region of rat aromatase mRNA. This probe protects two RNA fragments in rat brain tissue: a 430-nt length fragment and a shorter 300-nt fragment. The presence of the 300-nt RNA fragment is not associated with enzyme activity in the rat brain and appears to represent an alternative brain-specific aromatase transcript whose function, if any, is unknown. In contrast, the 430-nt RNA fragment represents mRNA, which is thought to encode functional aromatase enzyme because its levels are correlated with aromatase activity concentrations in preoptic area, hypothalamus, amygdala, and ovary. Aromatase activity and mRNA levels in the preoptic area and hypothalamus decreased by 7 days after castration and were maintained at intact levels by treatment with testosterone and dihyhdrotestosterone, but not with estradiol. In contrast, neither aromatase activity nor mRNA levels in the amygdala are affected by castration or hormone replacement. In addition, sex differences in the regulation of aromatase mRNA were apparent in both the preoptic area and hypothalamus. These results demonstrate that androgens regulate the transcription or stability of aromatase mRNA in specific brain areas. Moreover, they suggest that gender differences in androgen responsiveness play an important role in regulating gene expression in the adult rat brain.",
keywords = "Amygdala, Aromatase, MRNA, Preoptic area, Rat brain, Sex differences",
author = "Charles Roselli and Abdelgadir, {Salah E.} and Resko, {John A.}",
year = "1997",
doi = "10.1016/S0361-9230(97)00214-1",
language = "English (US)",
volume = "44",
pages = "351--357",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Regulation of aromatase gene expression in the adult rat brain

AU - Roselli, Charles

AU - Abdelgadir, Salah E.

AU - Resko, John A.

PY - 1997

Y1 - 1997

N2 - Brain aromatase plays an important role in the regulation of adult reproductive behavior in male rodents. This report focuses on recent experiments from our laboratory that examined the distribution and regulation of aromatase mRNA in the rat brain. Aromatase mRNA was measured by a highly sensitive ribonuclease protection assay using a 32P-labeled antisense RNA probe that was complimentary to the 5' coding region of rat aromatase mRNA. This probe protects two RNA fragments in rat brain tissue: a 430-nt length fragment and a shorter 300-nt fragment. The presence of the 300-nt RNA fragment is not associated with enzyme activity in the rat brain and appears to represent an alternative brain-specific aromatase transcript whose function, if any, is unknown. In contrast, the 430-nt RNA fragment represents mRNA, which is thought to encode functional aromatase enzyme because its levels are correlated with aromatase activity concentrations in preoptic area, hypothalamus, amygdala, and ovary. Aromatase activity and mRNA levels in the preoptic area and hypothalamus decreased by 7 days after castration and were maintained at intact levels by treatment with testosterone and dihyhdrotestosterone, but not with estradiol. In contrast, neither aromatase activity nor mRNA levels in the amygdala are affected by castration or hormone replacement. In addition, sex differences in the regulation of aromatase mRNA were apparent in both the preoptic area and hypothalamus. These results demonstrate that androgens regulate the transcription or stability of aromatase mRNA in specific brain areas. Moreover, they suggest that gender differences in androgen responsiveness play an important role in regulating gene expression in the adult rat brain.

AB - Brain aromatase plays an important role in the regulation of adult reproductive behavior in male rodents. This report focuses on recent experiments from our laboratory that examined the distribution and regulation of aromatase mRNA in the rat brain. Aromatase mRNA was measured by a highly sensitive ribonuclease protection assay using a 32P-labeled antisense RNA probe that was complimentary to the 5' coding region of rat aromatase mRNA. This probe protects two RNA fragments in rat brain tissue: a 430-nt length fragment and a shorter 300-nt fragment. The presence of the 300-nt RNA fragment is not associated with enzyme activity in the rat brain and appears to represent an alternative brain-specific aromatase transcript whose function, if any, is unknown. In contrast, the 430-nt RNA fragment represents mRNA, which is thought to encode functional aromatase enzyme because its levels are correlated with aromatase activity concentrations in preoptic area, hypothalamus, amygdala, and ovary. Aromatase activity and mRNA levels in the preoptic area and hypothalamus decreased by 7 days after castration and were maintained at intact levels by treatment with testosterone and dihyhdrotestosterone, but not with estradiol. In contrast, neither aromatase activity nor mRNA levels in the amygdala are affected by castration or hormone replacement. In addition, sex differences in the regulation of aromatase mRNA were apparent in both the preoptic area and hypothalamus. These results demonstrate that androgens regulate the transcription or stability of aromatase mRNA in specific brain areas. Moreover, they suggest that gender differences in androgen responsiveness play an important role in regulating gene expression in the adult rat brain.

KW - Amygdala

KW - Aromatase

KW - MRNA

KW - Preoptic area

KW - Rat brain

KW - Sex differences

UR - http://www.scopus.com/inward/record.url?scp=0030726669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030726669&partnerID=8YFLogxK

U2 - 10.1016/S0361-9230(97)00214-1

DO - 10.1016/S0361-9230(97)00214-1

M3 - Article

C2 - 9370199

AN - SCOPUS:0030726669

VL - 44

SP - 351

EP - 357

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 4

ER -