Regulated expression of the neurofibromin type I transcript in the developing chicken brain

Lawrence Baizer, Gary Ciment, Susan K. Hendrickson, Gwen L. Schafer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Neurofibromatosis type 1 (NF-1) is among the most common inherited diseases affecting cells of the central and peripheral nervous systems. A region of the NF-1 gene is similar in sequence to the ras-GTPase activator protein (ras-GAP), and investigations have confirmed that the NF-1 gene product (now known as neurofibromin) stimulates ras-GTPase activity in vitro and in vivo. Neurofibromin modulates the ability of ras proteins to regulate cellular proliferation and/or differentiation, suggesting a possible role in normal development. An alternative form of the neurofibromin transcript with an additional 63-bp exon inserted in the GAP-related domain (GRD) has been described recently. To determine whether differential expression of the two forms of neurofibromin GRD mRNA plays a role in embryonic development, we have isolated and characterized the corresponding chicken cDNA. The predicted amino acid sequence for the inserted exon is identical between chick and human, as are the exon-intron boundaries. RNase protection and RNA-polymerase chain reaction analyses demonstrate that most tissues express predominantly type II mRNA (which contains the insert) throughout embryonic development. In contrast, whereas type II is the major form in the brain early in development, expression of the type I transcript (without the insert) in this tissue increases dramatically at later times. Analysis of primary cultures derived from chick embryo brain indicates that the type I mRNA is enriched in neurons.

Original languageEnglish (US)
Pages (from-to)2054-2060
Number of pages7
JournalJournal of Neurochemistry
Volume61
Issue number6
StatePublished - Dec 1993

Fingerprint

Neurofibromin 1
Chickens
Brain
ras Proteins
Exons
Neurofibromatosis 1
Messenger RNA
Embryonic Development
GTP Phosphohydrolase Activators
Neurofibromatosis 1 Genes
Tissue
Polymerase chain reaction
Peripheral Nervous System
Neurology
Chick Embryo
DNA-Directed RNA Polymerases
Ribonucleases
Introns
Neurons
Amino Acid Sequence

Keywords

  • mRNA splicing
  • Neurofibromatosis
  • Neuronal development

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Baizer, L., Ciment, G., Hendrickson, S. K., & Schafer, G. L. (1993). Regulated expression of the neurofibromin type I transcript in the developing chicken brain. Journal of Neurochemistry, 61(6), 2054-2060.

Regulated expression of the neurofibromin type I transcript in the developing chicken brain. / Baizer, Lawrence; Ciment, Gary; Hendrickson, Susan K.; Schafer, Gwen L.

In: Journal of Neurochemistry, Vol. 61, No. 6, 12.1993, p. 2054-2060.

Research output: Contribution to journalArticle

Baizer, L, Ciment, G, Hendrickson, SK & Schafer, GL 1993, 'Regulated expression of the neurofibromin type I transcript in the developing chicken brain', Journal of Neurochemistry, vol. 61, no. 6, pp. 2054-2060.
Baizer, Lawrence ; Ciment, Gary ; Hendrickson, Susan K. ; Schafer, Gwen L. / Regulated expression of the neurofibromin type I transcript in the developing chicken brain. In: Journal of Neurochemistry. 1993 ; Vol. 61, No. 6. pp. 2054-2060.
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