Regional expression of prostaglandin E2 and F2alpha receptors in human myometrium, amnion, and choriodecidua with advancing gestation and labor

Peta Grigsby, Suren R. Sooranna, Bernice Adu-Amankwa, Brad Pitzer, Diane E. Brockman, Mark R. Johnson, Leslie Myatt

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The change from uterine quiescence to enhanced contractile activity may be due to the differential expression of prostaglandin receptors within the myometrium and fetal membranes, in a temporal and topographically distinct manner. To address this question, we determined the localization and expression of the PGE2 receptor subtypes (PTGER1-4) and the PGF2alpha receptor (PTGFR) in paired upper and lower segment myometrium, amnion, and choriodecidual samples throughout human pregnancy, with and without labor. All receptor subtypes were found throughout the muscle layers in both the upper and lower uterine segments, colocalizing with alpha smooth muscle actin. A change in intracellular localization was observed at term labor, where PTGER1 and PTGER4 were predominately associated with the nucleus. Minimal changes in the expression of the PGE2 and PGF2alpha receptor subtypes were observed with gestational age, labor, or between the upper and lower myometrial segments. Receptor expression in maternal and fetal tissues differed between the receptor subtypes; PTGER1 and PTGER4 were predominately expressed in the fetal membranes, PTGER2 was greatest in the myometrium, whereas PTGER3 and PTGFR were similarly expressed in the myometrium and fetal membranes. Myometrial activation through the prostaglandin receptors is perhaps more subtle and may be mediated by a balance between one or several of the prostaglandin receptor subtypes together with other known contraction associated proteins. Lack of coordination in receptor expression between the myometrium and fetal membranes may indicate different regulatory mechanisms between these tissues, or it may suggest a function for these receptors in the amnion and choriodecidua that is independent of that seen in the myometrium.

Original languageEnglish (US)
Pages (from-to)297-305
Number of pages9
JournalBiology of Reproduction
Volume75
Issue number2
DOIs
StatePublished - Aug 2006
Externally publishedYes

Fingerprint

Amnion
Myometrium
Dinoprostone
Extraembryonic Membranes
Prostaglandin Receptors
Pregnancy
Prostaglandin E Receptors
Ataxia
Gestational Age
Smooth Muscle
prostaglandin F2alpha receptor
Actins
Fetus
Mothers
Muscles
Proteins

Keywords

  • Fetal membranes
  • Mechanisms of hormone action
  • Myometrium
  • Parturition
  • Prostaglandin receptors
  • Steroid hormone receptors
  • Steroid hormones

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Regional expression of prostaglandin E2 and F2alpha receptors in human myometrium, amnion, and choriodecidua with advancing gestation and labor. / Grigsby, Peta; Sooranna, Suren R.; Adu-Amankwa, Bernice; Pitzer, Brad; Brockman, Diane E.; Johnson, Mark R.; Myatt, Leslie.

In: Biology of Reproduction, Vol. 75, No. 2, 08.2006, p. 297-305.

Research output: Contribution to journalArticle

Grigsby, Peta ; Sooranna, Suren R. ; Adu-Amankwa, Bernice ; Pitzer, Brad ; Brockman, Diane E. ; Johnson, Mark R. ; Myatt, Leslie. / Regional expression of prostaglandin E2 and F2alpha receptors in human myometrium, amnion, and choriodecidua with advancing gestation and labor. In: Biology of Reproduction. 2006 ; Vol. 75, No. 2. pp. 297-305.
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