Regional expression of mRNA encoding a second form of gonadotropin- releasing hormone in the Macaque brain

Henryk Urbanski, Richard B. White, Russell D. Fernald, Steven Kohama, Vasilios T. Garyfallou, Valerie S. Densmore

    Research output: Contribution to journalArticle

    97 Citations (Scopus)

    Abstract

    In mammals, reproduction is thought to be controlled by a single neuropeptide, gonadotropin-releasing hormone (GnRH-I), which regulates the synthesis and secretion of gonadotropins from the pituitary gland. However, another form of this decapeptide (GnRH-II), of unknown function, also exists in the brain of many vertebrate species, including humans; it is encoded by a different gene and its amino acid sequence is 70% identical to that of GnRH- I. Here we report the cloning of a GnRH-II cDNA from the rhesus macaque (Macaca mulatta), and show for the first time by in situ hybridization that GnRH-II mRNA is expressed in the primate midbrain, hippocampus and discrete nuclei of the hypothalamus, including the supraoptic, paraventricular, suprachiasmatic and arcuate. Because the regional distribution pattern of cells containing GnRH-II mRNA is largely dissimilar to that of cells containing GnRH-I mRNA, it is likely that these two cell populations receive distinct neuroendocrine inputs and thus regulate GnRH synthesis and release differently.

    Original languageEnglish (US)
    Pages (from-to)1945-1948
    Number of pages4
    JournalEndocrinology
    Volume140
    Issue number4
    StatePublished - 1999

    Fingerprint

    Macaca
    Gonadotropin-Releasing Hormone
    Messenger RNA
    Brain
    Macaca mulatta
    Supraoptic Nucleus
    Pituitary Gland
    Mesencephalon
    Neuropeptides
    Gonadotropins
    Primates
    In Situ Hybridization
    Reproduction
    Vertebrates
    Organism Cloning
    Mammals
    Amino Acid Sequence
    Hippocampus
    Complementary DNA
    His(5)-Trp(7)-Tyr(8)-lHRH

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Urbanski, H., White, R. B., Fernald, R. D., Kohama, S., Garyfallou, V. T., & Densmore, V. S. (1999). Regional expression of mRNA encoding a second form of gonadotropin- releasing hormone in the Macaque brain. Endocrinology, 140(4), 1945-1948.

    Regional expression of mRNA encoding a second form of gonadotropin- releasing hormone in the Macaque brain. / Urbanski, Henryk; White, Richard B.; Fernald, Russell D.; Kohama, Steven; Garyfallou, Vasilios T.; Densmore, Valerie S.

    In: Endocrinology, Vol. 140, No. 4, 1999, p. 1945-1948.

    Research output: Contribution to journalArticle

    Urbanski, H, White, RB, Fernald, RD, Kohama, S, Garyfallou, VT & Densmore, VS 1999, 'Regional expression of mRNA encoding a second form of gonadotropin- releasing hormone in the Macaque brain', Endocrinology, vol. 140, no. 4, pp. 1945-1948.
    Urbanski H, White RB, Fernald RD, Kohama S, Garyfallou VT, Densmore VS. Regional expression of mRNA encoding a second form of gonadotropin- releasing hormone in the Macaque brain. Endocrinology. 1999;140(4):1945-1948.
    Urbanski, Henryk ; White, Richard B. ; Fernald, Russell D. ; Kohama, Steven ; Garyfallou, Vasilios T. ; Densmore, Valerie S. / Regional expression of mRNA encoding a second form of gonadotropin- releasing hormone in the Macaque brain. In: Endocrinology. 1999 ; Vol. 140, No. 4. pp. 1945-1948.
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