Regional distribution of glutamic acid decarboxylase (GAD65 and GAD67) mRNA in the hypothalamus of male rhesus macaques before and after puberty

Henryk Urbanski, Sarina M. Rodrigues, Vasilios T. Garyfallou, Steven Kohama

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    Glutamic acid decarboxylase (GAD) is the rate-limiting enzyme in the γ-aminobutyric acid (GABA) biosynthetic pathway, and is coded for by two mRNAs, GAD65 and GAD67. Using in situ hybridization, we examine the distribution pattern of both GAD mRNAs in the hypothalamus and thalamus of prepubertal and adult male rhesus macaques. Qualitatively, GAD65 and GAD67 mRNAs showed a similar wide, but highly specific distribution pattern, supporting the view that GABAergic neurons play an important role in modulating neuroendocrine function. However, no quantitative difference in the intensity of hybridization signal was detected between prepubertal and adult animals in any of the hypothalamic or thalamic nuclei. Therefore, although GABAergic neurons are anatomically well-placed to control the secretion of gonadotropin-releasing hormone (GnRH) in primates, it is unlikely that the onset of puberty and the associated increase in GnRH secretion is triggered by a change in GAD gene transcription.

    Original languageEnglish (US)
    Pages (from-to)86-91
    Number of pages6
    JournalMolecular Brain Research
    Volume57
    Issue number1
    DOIs
    StatePublished - Jun 1 1998

    Fingerprint

    Glutamate Decarboxylase
    Puberty
    Macaca mulatta
    Hypothalamus
    GABAergic Neurons
    Gonadotropin-Releasing Hormone
    Messenger RNA
    Aminobutyrates
    Thalamic Nuclei
    Biosynthetic Pathways
    Thalamus
    gamma-Aminobutyric Acid
    Primates
    In Situ Hybridization
    Enzymes
    Genes

    Keywords

    • γ-aminobutyric acid
    • Gonadotropin-releasing hormone
    • In situ hybridization

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience

    Cite this

    Regional distribution of glutamic acid decarboxylase (GAD65 and GAD67) mRNA in the hypothalamus of male rhesus macaques before and after puberty. / Urbanski, Henryk; Rodrigues, Sarina M.; Garyfallou, Vasilios T.; Kohama, Steven.

    In: Molecular Brain Research, Vol. 57, No. 1, 01.06.1998, p. 86-91.

    Research output: Contribution to journalArticle

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