Regional distribution of free calcium in selenite cataract

Relation to calpain II

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The purpose of this experiment was to assess the roles of free, intracellular calcium and calcium-dependent neutral protease (calpain II, EC.34.22.17) in selenite nuclear cataract. Free calcium ion concentrations within lens nuclear fibers during selenite cataractogenesis increased to 3 μM on day 2 post-injection (clear lens) and to 108 μM at day 4 (nuclear cataract). Calpain II is known to be activated in vitro by calcium levels above 50 μM. Calpain II activity was present in the lens nucleus at time periods preceding formation of selenite cataract. These data suggested that after selenite injection, calpain II was activated by elevated free calcium in the nucleus, and that calpain II-induced proteolysis of nuclear proteins was an important mechanism in selenite cataract. Calpain II levels were also observed to decrease in the nucleus during selenite cataractogenesis, probably due to autolysis. This was supported by the finding that incubation of purified lens calpain II with 100 μM calcium caused partial inactivation of the protease.

Original languageEnglish (US)
Pages (from-to)1702-1706
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume28
Issue number10
StatePublished - 1987
Externally publishedYes

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Calpain
Selenious Acid
Cataract
Lenses
Calcium
Autolysis
Injections
calcium selenite
Nuclear Proteins
Proteolysis
Peptide Hydrolases
Ions

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Regional distribution of free calcium in selenite cataract : Relation to calpain II. / Hightower, K. R.; David, Larry; Shearer, Thomas (Tom).

In: Investigative Ophthalmology and Visual Science, Vol. 28, No. 10, 1987, p. 1702-1706.

Research output: Contribution to journalArticle

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abstract = "The purpose of this experiment was to assess the roles of free, intracellular calcium and calcium-dependent neutral protease (calpain II, EC.34.22.17) in selenite nuclear cataract. Free calcium ion concentrations within lens nuclear fibers during selenite cataractogenesis increased to 3 μM on day 2 post-injection (clear lens) and to 108 μM at day 4 (nuclear cataract). Calpain II is known to be activated in vitro by calcium levels above 50 μM. Calpain II activity was present in the lens nucleus at time periods preceding formation of selenite cataract. These data suggested that after selenite injection, calpain II was activated by elevated free calcium in the nucleus, and that calpain II-induced proteolysis of nuclear proteins was an important mechanism in selenite cataract. Calpain II levels were also observed to decrease in the nucleus during selenite cataractogenesis, probably due to autolysis. This was supported by the finding that incubation of purified lens calpain II with 100 μM calcium caused partial inactivation of the protease.",
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