TY - JOUR
T1 - Regional and cellular distribution of protein kinase C in rat cerebellar Purkinje cells
AU - Barmack, Neal H.
AU - Qian, Zuyuan
AU - Yoshimura, Jason
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000/11/13
Y1 - 2000/11/13
N2 - Protein kinase C (PCK) is a family of isoforms that are implicated in subcellular signal transduction. The authors investigated the distribution of several PKC isoforms (PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε) within major cerebellar cell types as well as cerebellar projection target neurons, including Purkinje neurons, cerebellar nuclear neurons, and secondary vestibular neurons. PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε are found within the cerebellum. Of these isoforms, PKC-γ and PKC-δ are highly expressed in Purkinje cells. PKC-γ is expressed in all Purkinje cells, whereas the expression of PKC-δ is restricted to sagittal bands of Purkinje cells in the posterior cerebellar cortex. In the lower folia of the uvula and nodulus, Purkinje cell expression of PKC-δ is uniformly high, and the sagittal banding for PKC-δ expression is absent. Within the cerebellar nuclei, PKC-δ-immunolabeled axons terminate within the medial aspect of the caudal half of the ipsilateral interpositus nucleus. PKC δ-immunolabeled axons also terminated within the caudal medial and descending vestibular nuclei (MVN and DVN, respectively), the parasolitary nucleus (Psol), and the nucleus prepositus hypoglossi (NPH). PKC-γ-immunolabeled axons terminated in all of the cerebellar nuclei as well as in the lateral and superior vestibular nuclei and the MVN, DVN, Psol, and NPH. The projection patterns of PKC-immunolabeled Purkinje cells were confirmed by lesion-depletion studies in which unilateral uvula-nodular lesions caused depletion of PKC-immunolabeled terminals ipsilateral to the lesion in the vestibular complex. These data identify circuitry that is unique to cerebellar-vestibular interactions. (C) 2000 Wiley-Liss, Inc.
AB - Protein kinase C (PCK) is a family of isoforms that are implicated in subcellular signal transduction. The authors investigated the distribution of several PKC isoforms (PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε) within major cerebellar cell types as well as cerebellar projection target neurons, including Purkinje neurons, cerebellar nuclear neurons, and secondary vestibular neurons. PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε are found within the cerebellum. Of these isoforms, PKC-γ and PKC-δ are highly expressed in Purkinje cells. PKC-γ is expressed in all Purkinje cells, whereas the expression of PKC-δ is restricted to sagittal bands of Purkinje cells in the posterior cerebellar cortex. In the lower folia of the uvula and nodulus, Purkinje cell expression of PKC-δ is uniformly high, and the sagittal banding for PKC-δ expression is absent. Within the cerebellar nuclei, PKC-δ-immunolabeled axons terminate within the medial aspect of the caudal half of the ipsilateral interpositus nucleus. PKC δ-immunolabeled axons also terminated within the caudal medial and descending vestibular nuclei (MVN and DVN, respectively), the parasolitary nucleus (Psol), and the nucleus prepositus hypoglossi (NPH). PKC-γ-immunolabeled axons terminated in all of the cerebellar nuclei as well as in the lateral and superior vestibular nuclei and the MVN, DVN, Psol, and NPH. The projection patterns of PKC-immunolabeled Purkinje cells were confirmed by lesion-depletion studies in which unilateral uvula-nodular lesions caused depletion of PKC-immunolabeled terminals ipsilateral to the lesion in the vestibular complex. These data identify circuitry that is unique to cerebellar-vestibular interactions. (C) 2000 Wiley-Liss, Inc.
KW - Cerebellar nuclei
KW - Cerebellum
KW - Descending vestibular nucleus
KW - Medial vestibular nucleus
KW - Nodulus
KW - Nucleus prepositus hypoglossi
KW - Parasolitary nucleus
KW - Uvula
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U2 - 10.1002/1096-9861(20001113)427:2<235::AID-CNE6>3.0.CO;2-6
DO - 10.1002/1096-9861(20001113)427:2<235::AID-CNE6>3.0.CO;2-6
M3 - Article
C2 - 11054691
AN - SCOPUS:0034645382
SN - 0021-9967
VL - 427
SP - 235
EP - 254
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 2
ER -