Regional and cellular distribution of protein kinase C in rat cerebellar Purkinje cells

Neal H. Barmack, Zuyuan Qian, Jason Yoshimura

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Protein kinase C (PCK) is a family of isoforms that are implicated in subcellular signal transduction. The authors investigated the distribution of several PKC isoforms (PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε) within major cerebellar cell types as well as cerebellar projection target neurons, including Purkinje neurons, cerebellar nuclear neurons, and secondary vestibular neurons. PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε are found within the cerebellum. Of these isoforms, PKC-γ and PKC-δ are highly expressed in Purkinje cells. PKC-γ is expressed in all Purkinje cells, whereas the expression of PKC-δ is restricted to sagittal bands of Purkinje cells in the posterior cerebellar cortex. In the lower folia of the uvula and nodulus, Purkinje cell expression of PKC-δ is uniformly high, and the sagittal banding for PKC-δ expression is absent. Within the cerebellar nuclei, PKC-δ-immunolabeled axons terminate within the medial aspect of the caudal half of the ipsilateral interpositus nucleus. PKC δ-immunolabeled axons also terminated within the caudal medial and descending vestibular nuclei (MVN and DVN, respectively), the parasolitary nucleus (Psol), and the nucleus prepositus hypoglossi (NPH). PKC-γ-immunolabeled axons terminated in all of the cerebellar nuclei as well as in the lateral and superior vestibular nuclei and the MVN, DVN, Psol, and NPH. The projection patterns of PKC-immunolabeled Purkinje cells were confirmed by lesion-depletion studies in which unilateral uvula-nodular lesions caused depletion of PKC-immunolabeled terminals ipsilateral to the lesion in the vestibular complex. These data identify circuitry that is unique to cerebellar-vestibular interactions. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)235-254
Number of pages20
JournalJournal of Comparative Neurology
Volume427
Issue number2
DOIs
StatePublished - Nov 13 2000

Fingerprint

Purkinje Cells
Protein Kinase C
Uvula
Axons
Cerebellar Nuclei
Protein Isoforms
Neurons
Lateral Vestibular Nucleus
Vestibular Nuclei
Cerebellar Cortex
Cerebellum
Signal Transduction

Keywords

  • Cerebellar nuclei
  • Cerebellum
  • Descending vestibular nucleus
  • Medial vestibular nucleus
  • Nodulus
  • Nucleus prepositus hypoglossi
  • Parasolitary nucleus
  • Uvula

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regional and cellular distribution of protein kinase C in rat cerebellar Purkinje cells. / Barmack, Neal H.; Qian, Zuyuan; Yoshimura, Jason.

In: Journal of Comparative Neurology, Vol. 427, No. 2, 13.11.2000, p. 235-254.

Research output: Contribution to journalArticle

Barmack, Neal H. ; Qian, Zuyuan ; Yoshimura, Jason. / Regional and cellular distribution of protein kinase C in rat cerebellar Purkinje cells. In: Journal of Comparative Neurology. 2000 ; Vol. 427, No. 2. pp. 235-254.
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AU - Barmack, Neal H.

AU - Qian, Zuyuan

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N2 - Protein kinase C (PCK) is a family of isoforms that are implicated in subcellular signal transduction. The authors investigated the distribution of several PKC isoforms (PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε) within major cerebellar cell types as well as cerebellar projection target neurons, including Purkinje neurons, cerebellar nuclear neurons, and secondary vestibular neurons. PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε are found within the cerebellum. Of these isoforms, PKC-γ and PKC-δ are highly expressed in Purkinje cells. PKC-γ is expressed in all Purkinje cells, whereas the expression of PKC-δ is restricted to sagittal bands of Purkinje cells in the posterior cerebellar cortex. In the lower folia of the uvula and nodulus, Purkinje cell expression of PKC-δ is uniformly high, and the sagittal banding for PKC-δ expression is absent. Within the cerebellar nuclei, PKC-δ-immunolabeled axons terminate within the medial aspect of the caudal half of the ipsilateral interpositus nucleus. PKC δ-immunolabeled axons also terminated within the caudal medial and descending vestibular nuclei (MVN and DVN, respectively), the parasolitary nucleus (Psol), and the nucleus prepositus hypoglossi (NPH). PKC-γ-immunolabeled axons terminated in all of the cerebellar nuclei as well as in the lateral and superior vestibular nuclei and the MVN, DVN, Psol, and NPH. The projection patterns of PKC-immunolabeled Purkinje cells were confirmed by lesion-depletion studies in which unilateral uvula-nodular lesions caused depletion of PKC-immunolabeled terminals ipsilateral to the lesion in the vestibular complex. These data identify circuitry that is unique to cerebellar-vestibular interactions. (C) 2000 Wiley-Liss, Inc.

AB - Protein kinase C (PCK) is a family of isoforms that are implicated in subcellular signal transduction. The authors investigated the distribution of several PKC isoforms (PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε) within major cerebellar cell types as well as cerebellar projection target neurons, including Purkinje neurons, cerebellar nuclear neurons, and secondary vestibular neurons. PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε are found within the cerebellum. Of these isoforms, PKC-γ and PKC-δ are highly expressed in Purkinje cells. PKC-γ is expressed in all Purkinje cells, whereas the expression of PKC-δ is restricted to sagittal bands of Purkinje cells in the posterior cerebellar cortex. In the lower folia of the uvula and nodulus, Purkinje cell expression of PKC-δ is uniformly high, and the sagittal banding for PKC-δ expression is absent. Within the cerebellar nuclei, PKC-δ-immunolabeled axons terminate within the medial aspect of the caudal half of the ipsilateral interpositus nucleus. PKC δ-immunolabeled axons also terminated within the caudal medial and descending vestibular nuclei (MVN and DVN, respectively), the parasolitary nucleus (Psol), and the nucleus prepositus hypoglossi (NPH). PKC-γ-immunolabeled axons terminated in all of the cerebellar nuclei as well as in the lateral and superior vestibular nuclei and the MVN, DVN, Psol, and NPH. The projection patterns of PKC-immunolabeled Purkinje cells were confirmed by lesion-depletion studies in which unilateral uvula-nodular lesions caused depletion of PKC-immunolabeled terminals ipsilateral to the lesion in the vestibular complex. These data identify circuitry that is unique to cerebellar-vestibular interactions. (C) 2000 Wiley-Liss, Inc.

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KW - Uvula

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