Region- and cell type-specific de novo DNA methylation in cultured mammalian cells

Mitchell Turker, Padmaja Mummaneni, Peggy L. Bishop

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A region upstream of the mouse adenine phosphoribosyltransferase (aprt) gene has a well characterized methylation pattern for HpaII/MspI sites. When an unmethylated plasmid construct containing this region was transfected into P19 mouse teratocarcinoma stem cells appropriate de novo methylation was observed. However, de novo methylation was significantly reduced when this plasmid was introduced into a differentiated derivative of the P19 stem cell line. Finally, a position effect for de novo methylation was shown by demonstrating methylation of a normally unmethylated HpaII/MspI site when it was placed in this upstream region. This system should prove useful for elucidating DNA signals for de novo methylation and changes in DNA methyltransferase activities that occur during cellular differentiation.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalSomatic Cell and Molecular Genetics
Volume17
Issue number2
DOIs
StatePublished - Mar 1991
Externally publishedYes

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DNA Methylation
Methylation
Cultured Cells
Plasmids
Adenine Phosphoribosyltransferase
Embryonal Carcinoma Stem Cells
DNA
Methyltransferases
Stem Cells
Cell Line
Genes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Region- and cell type-specific de novo DNA methylation in cultured mammalian cells. / Turker, Mitchell; Mummaneni, Padmaja; Bishop, Peggy L.

In: Somatic Cell and Molecular Genetics, Vol. 17, No. 2, 03.1991, p. 151-157.

Research output: Contribution to journalArticle

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