TY - JOUR
T1 - Reflectance confocal microscopy of optical phantoms
AU - Jacques, Steven L.
AU - Wang, Bo
AU - Samatham, Ravikant
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2012/6/1
Y1 - 2012/6/1
N2 - A reflectance confocal scanning laser microscope (rCSLM) operating at 488-nm wavelength imaged three types of optical phantoms: (1) 100-nm-dia. polystyrene microspheres in gel at 2% volume fraction, (2) solid polyurethane phantoms (INO Biomimic™), and (3) common reflectance standards (Spectralon™). The noninvasive method measured the exponential decay of reflected signal as the focus (zf) moved deeper into the material. The two experimental values, the attenuation coefficient μ and the pre-exponential factor ρ, were mapped into the material optical scattering properties, the scattering coefficient μs and the anisotropy of scattering g. Results show that μs varies as 58, 8-24, and 130-200 cm-1 for phantom types (1), (2) and (3), respectively. The g varies as 0.112, 0.53-0.67, and 0.003-0.26, respectively.
AB - A reflectance confocal scanning laser microscope (rCSLM) operating at 488-nm wavelength imaged three types of optical phantoms: (1) 100-nm-dia. polystyrene microspheres in gel at 2% volume fraction, (2) solid polyurethane phantoms (INO Biomimic™), and (3) common reflectance standards (Spectralon™). The noninvasive method measured the exponential decay of reflected signal as the focus (zf) moved deeper into the material. The two experimental values, the attenuation coefficient μ and the pre-exponential factor ρ, were mapped into the material optical scattering properties, the scattering coefficient μs and the anisotropy of scattering g. Results show that μs varies as 58, 8-24, and 130-200 cm-1 for phantom types (1), (2) and (3), respectively. The g varies as 0.112, 0.53-0.67, and 0.003-0.26, respectively.
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U2 - 10.1364/BOE.3.001162
DO - 10.1364/BOE.3.001162
M3 - Article
C2 - 22741065
AN - SCOPUS:84863953092
VL - 3
SP - 1162
EP - 1172
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 6
ER -