Reexpression of the retinoblastoma protein in tumor cells induces senescence and telomerase inhibition

Hong Ji Xu, Yunli Zhou, Wan Ji, Guang Shing Perng, Russell Kruzelock, Cheng Te Kong, Robert C. Bast, Gordon Mills, Jian Li, Shi Xue Hu

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Normal human diploid cells senesce in vitro and in vivo after a limited number of cell divisions. This process known as cellular senescence is an underlying cause of aging and a critical barrier for development of human cancers. We demonstrate here that reexpression of functional pRB alone in RB/p53-defective tumor cells via a modified tetracycline-regulated gene expression system resulted in a stable growth arrest at the G0/G1 phase of the cell cycle, preventing tumor cells from entering S phase in response to a variety of mitogenic stimuli. These cells displayed multiple morphological changes consistent with cellular senescence and expressed a senescence-associated β-galactosidase biomarker. Further studies indicated that telomerase activity, which was assumably essential for an extended proliferative life-span of neoplastic cells, was abrogated or repressed in the tumor cell lines after induction of pRB (but not p53) expression. Strikingly, when returned to an non-permissive medium for pRB expression, the pRB-induced senescent tumor cells resumed DNA synthesis, attempted to divide but most died in the process, a phenomenon similar to postsenescent crisis of SV40 T-antigen-transformed human diploid fibroblasts in late passage. These observations provide direct evidence that over-expression of pRB alone in RB/p53-defective tumor cells is sufficient to reverse their immortality and cause a phenotype that is, by all generally accepted criteria, indistinguishable from replicative senescence. The results suggest that pRB may play a causal role in the intrinsic cellular senescence program.

Original languageEnglish (US)
Pages (from-to)2589-2596
Number of pages8
JournalOncogene
Volume15
Issue number21
StatePublished - Dec 9 1997
Externally publishedYes

Fingerprint

Retinoblastoma Protein
Cell Aging
Telomerase
Neoplasms
Diploidy
Galactosidases
Polyomavirus Transforming Antigens
Cell Cycle Resting Phase
G1 Phase
Human Development
Tetracycline
Tumor Cell Line
S Phase
Cell Division
Inhibition (Psychology)
Cell Cycle
Fibroblasts
Biomarkers
Phenotype
Gene Expression

Keywords

  • p53
  • Retinoblastoma (RB) gene
  • Senescence
  • Telomerase
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Xu, H. J., Zhou, Y., Ji, W., Perng, G. S., Kruzelock, R., Kong, C. T., ... Hu, S. X. (1997). Reexpression of the retinoblastoma protein in tumor cells induces senescence and telomerase inhibition. Oncogene, 15(21), 2589-2596.

Reexpression of the retinoblastoma protein in tumor cells induces senescence and telomerase inhibition. / Xu, Hong Ji; Zhou, Yunli; Ji, Wan; Perng, Guang Shing; Kruzelock, Russell; Kong, Cheng Te; Bast, Robert C.; Mills, Gordon; Li, Jian; Hu, Shi Xue.

In: Oncogene, Vol. 15, No. 21, 09.12.1997, p. 2589-2596.

Research output: Contribution to journalArticle

Xu, HJ, Zhou, Y, Ji, W, Perng, GS, Kruzelock, R, Kong, CT, Bast, RC, Mills, G, Li, J & Hu, SX 1997, 'Reexpression of the retinoblastoma protein in tumor cells induces senescence and telomerase inhibition', Oncogene, vol. 15, no. 21, pp. 2589-2596.
Xu HJ, Zhou Y, Ji W, Perng GS, Kruzelock R, Kong CT et al. Reexpression of the retinoblastoma protein in tumor cells induces senescence and telomerase inhibition. Oncogene. 1997 Dec 9;15(21):2589-2596.
Xu, Hong Ji ; Zhou, Yunli ; Ji, Wan ; Perng, Guang Shing ; Kruzelock, Russell ; Kong, Cheng Te ; Bast, Robert C. ; Mills, Gordon ; Li, Jian ; Hu, Shi Xue. / Reexpression of the retinoblastoma protein in tumor cells induces senescence and telomerase inhibition. In: Oncogene. 1997 ; Vol. 15, No. 21. pp. 2589-2596.
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