Reduction of central nervous system reperfusion injury in rabbits using doxycycline treatment

Wayne Clark, Frank A. Calcagno, Walter L. Gabler, John R. Smith, Bruce M. Coull

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background and Purpose Activated leukocytes appear to potentiate central nervous system reperfusion injury, and agents that block leukocyte adhesion have shown neuroprotec-tive efficacy in experimental models. Doxycycline, a tetracy-cline antibiotic, inhibits leukocyte function in vitro, presumably through divalent cation binding. We used a model of focal central nervous system reperfusion injury to determine the efficacy of doxycycline treatment in preserving neurological function. Methods Rabbits randomly received 10 mg/kg IV doxycycline 30 minutes before ischemia (pretreatment group) or 45 minutes after ischemia (posttreatment group) or received phosphate-buffered saline vehicle (control group) followed by 10 mg/kg q 8 hours times two. The average length of reversible spinal cord ischemia required to produce paraplegia (P50) at 18 hours was calculated for each group. Results For the control group (n= 13), the P50 was 22.8±2.2 minutes;for the pretreatment group (n=14), 35.5±2.4 minutes (P

Original languageEnglish (US)
Pages (from-to)1411-1415
Number of pages5
JournalStroke
Volume25
Issue number7
StatePublished - 1994
Externally publishedYes

Fingerprint

Nervous System Trauma
Doxycycline
Reperfusion Injury
Leukocytes
Central Nervous System
Rabbits
Ischemia
Spinal Cord Ischemia
Control Groups
Paraplegia
Divalent Cations
Theoretical Models
Phosphates
Anti-Bacterial Agents

Keywords

  • Cerebral ischemia
  • Doxycycline
  • Leukocytes
  • Reperfusion

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing
  • Neuroscience(all)

Cite this

Clark, W., Calcagno, F. A., Gabler, W. L., Smith, J. R., & Coull, B. M. (1994). Reduction of central nervous system reperfusion injury in rabbits using doxycycline treatment. Stroke, 25(7), 1411-1415.

Reduction of central nervous system reperfusion injury in rabbits using doxycycline treatment. / Clark, Wayne; Calcagno, Frank A.; Gabler, Walter L.; Smith, John R.; Coull, Bruce M.

In: Stroke, Vol. 25, No. 7, 1994, p. 1411-1415.

Research output: Contribution to journalArticle

Clark, W, Calcagno, FA, Gabler, WL, Smith, JR & Coull, BM 1994, 'Reduction of central nervous system reperfusion injury in rabbits using doxycycline treatment', Stroke, vol. 25, no. 7, pp. 1411-1415.
Clark W, Calcagno FA, Gabler WL, Smith JR, Coull BM. Reduction of central nervous system reperfusion injury in rabbits using doxycycline treatment. Stroke. 1994;25(7):1411-1415.
Clark, Wayne ; Calcagno, Frank A. ; Gabler, Walter L. ; Smith, John R. ; Coull, Bruce M. / Reduction of central nervous system reperfusion injury in rabbits using doxycycline treatment. In: Stroke. 1994 ; Vol. 25, No. 7. pp. 1411-1415.
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