Reduced mobilization of CD34+ stem cells in advanced human immunodeficiency virus type 1 disease

Robert T. Schooley, Jeannette Mladenovic, Anne Sevin, Simon Chiu, Steven A. Miles, Roger J. Pomerantz, Thomas B. Campbell, Dawn Bell, Daniel Ambruso, Robbie Wong, Alan Landay, Robert W. Coombs, Fox Lawrence, Malek Kamoun, Janice Jacovini

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Granulocyte colony-stimulating factor (r-met Hu G-CSF; filgrastim; 10 μg/kg/day for 7 days) was used to mobilize CD34+stem cells into the peripheral blood of human immunodeficiency virus type 1 (HIV-1)-infected individuals and a group of HIV-1-uninfected donors as a measure of immunologic reserve in HIV-1-infected people. G-CSF mobilized CD34+ cells of HIV-1-infected individuals with cell counts >500 CD4+ cells/mm3, as well as in HIV-1-uninfected donors. In contrast, CD34+ cell mobilization was significantly blunted in HIV-1-infected individuals with cell counts <500 CD4+ cells/mm3 (<200 cell days vs. >650 cell days, P < .0005, compared with the >500 CD4+ cell cohort). At least 1.75 x 107 CD34+ cells were harvested by leukapheresis from patients in each study cohort. CD34+ cell viability and the ability to differentiate precursor cells into myeloid and erythroid progenitor cells were not affected by HIV-1 infection.

Original languageEnglish (US)
Pages (from-to)148-157
Number of pages10
JournalJournal of Infectious Diseases
Volume181
Issue number1
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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