Reduced leukocyte migration, but normal rolling and arrest, in interleukin-8 receptor homologue knockout mice

Matthias D. Becker, Leslie M. O'Rourke, Whitney S. Blackman, Stephen Planck, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

PURPOSE. To determine the role of the murine interleukin-8 receptor homologue (mIL-8Rh, neutrophil chemokine CXC receptor 2) in leukocyte migration using intravital microscopy in a standardized model of eye inflammation, endotoxin-induced uveitis (EIU). METHODS. Two hundred fifty nanograms of E. coli endotoxin was injected into the vitreous of knockout mIL-8Rh(-/-) (n = 7) mice or heterozygous littermate mIL-8Rh(+/-) controls (n = 7). Intravital microscopic examination of iris microvasculature was performed at baseline and 6 and 24 hours after endotoxin injection. The numbers of rolling (cells/mm2 endothelial surface/min), sticking (cells/mm2 endothelial surface), and infiltrating cells (cells/mm2 iris tissue) were evaluated by digital off-line quantification. RESULTS. The number of infiltrating cells was significantly reduced in mIL-8Rh(-/-) mice: 406 ± 77 cells/mm2 at 6 hours and 242 ± 50 cells/mm2 at 24 hours in mIL-8Rh(+/-) mice versus 14 ± 4 cells/mm2 at 6 hours and 38 ± 11 cells/mm2 at 24 hours in mIL-8Rh(-/-) mice (P <0.001). In contrast, the absence of the IL-8 receptor homologue did not reduce rolling or sticking. CONCLUSIONS. Iris rhodamine angiography allows precise quantification of leukocyte endothelial dynamics in the absence of surgical trauma. IL-8 and its homologues are known to be potent signals for leukocyte migration. Although IL-8 has previously been implicated in cell adhesion, video imaging in vivo demonstrated that deletion of the IL-8 receptor homologue had minimal effect on rolling or arrest in this model of inflammation.

Original languageEnglish (US)
Pages (from-to)1812-1817
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume41
Issue number7
StatePublished - 2000

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Interleukin-8 Receptors
Knockout Mice
Leukocytes
Iris
Endotoxins
Interleukin-8
Interleukin-8B Receptors
Endothelial Cells
Cell Count
Inflammation
Rhodamines
Uveitis
Microvessels
Cell Adhesion
Angiography
Neutrophils
Escherichia coli
Injections
Wounds and Injuries

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Reduced leukocyte migration, but normal rolling and arrest, in interleukin-8 receptor homologue knockout mice. / Becker, Matthias D.; O'Rourke, Leslie M.; Blackman, Whitney S.; Planck, Stephen; Rosenbaum, James (Jim).

In: Investigative Ophthalmology and Visual Science, Vol. 41, No. 7, 2000, p. 1812-1817.

Research output: Contribution to journalArticle

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abstract = "PURPOSE. To determine the role of the murine interleukin-8 receptor homologue (mIL-8Rh, neutrophil chemokine CXC receptor 2) in leukocyte migration using intravital microscopy in a standardized model of eye inflammation, endotoxin-induced uveitis (EIU). METHODS. Two hundred fifty nanograms of E. coli endotoxin was injected into the vitreous of knockout mIL-8Rh(-/-) (n = 7) mice or heterozygous littermate mIL-8Rh(+/-) controls (n = 7). Intravital microscopic examination of iris microvasculature was performed at baseline and 6 and 24 hours after endotoxin injection. The numbers of rolling (cells/mm2 endothelial surface/min), sticking (cells/mm2 endothelial surface), and infiltrating cells (cells/mm2 iris tissue) were evaluated by digital off-line quantification. RESULTS. The number of infiltrating cells was significantly reduced in mIL-8Rh(-/-) mice: 406 ± 77 cells/mm2 at 6 hours and 242 ± 50 cells/mm2 at 24 hours in mIL-8Rh(+/-) mice versus 14 ± 4 cells/mm2 at 6 hours and 38 ± 11 cells/mm2 at 24 hours in mIL-8Rh(-/-) mice (P <0.001). In contrast, the absence of the IL-8 receptor homologue did not reduce rolling or sticking. CONCLUSIONS. Iris rhodamine angiography allows precise quantification of leukocyte endothelial dynamics in the absence of surgical trauma. IL-8 and its homologues are known to be potent signals for leukocyte migration. Although IL-8 has previously been implicated in cell adhesion, video imaging in vivo demonstrated that deletion of the IL-8 receptor homologue had minimal effect on rolling or arrest in this model of inflammation.",
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T1 - Reduced leukocyte migration, but normal rolling and arrest, in interleukin-8 receptor homologue knockout mice

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AU - Blackman, Whitney S.

AU - Planck, Stephen

AU - Rosenbaum, James (Jim)

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N2 - PURPOSE. To determine the role of the murine interleukin-8 receptor homologue (mIL-8Rh, neutrophil chemokine CXC receptor 2) in leukocyte migration using intravital microscopy in a standardized model of eye inflammation, endotoxin-induced uveitis (EIU). METHODS. Two hundred fifty nanograms of E. coli endotoxin was injected into the vitreous of knockout mIL-8Rh(-/-) (n = 7) mice or heterozygous littermate mIL-8Rh(+/-) controls (n = 7). Intravital microscopic examination of iris microvasculature was performed at baseline and 6 and 24 hours after endotoxin injection. The numbers of rolling (cells/mm2 endothelial surface/min), sticking (cells/mm2 endothelial surface), and infiltrating cells (cells/mm2 iris tissue) were evaluated by digital off-line quantification. RESULTS. The number of infiltrating cells was significantly reduced in mIL-8Rh(-/-) mice: 406 ± 77 cells/mm2 at 6 hours and 242 ± 50 cells/mm2 at 24 hours in mIL-8Rh(+/-) mice versus 14 ± 4 cells/mm2 at 6 hours and 38 ± 11 cells/mm2 at 24 hours in mIL-8Rh(-/-) mice (P <0.001). In contrast, the absence of the IL-8 receptor homologue did not reduce rolling or sticking. CONCLUSIONS. Iris rhodamine angiography allows precise quantification of leukocyte endothelial dynamics in the absence of surgical trauma. IL-8 and its homologues are known to be potent signals for leukocyte migration. Although IL-8 has previously been implicated in cell adhesion, video imaging in vivo demonstrated that deletion of the IL-8 receptor homologue had minimal effect on rolling or arrest in this model of inflammation.

AB - PURPOSE. To determine the role of the murine interleukin-8 receptor homologue (mIL-8Rh, neutrophil chemokine CXC receptor 2) in leukocyte migration using intravital microscopy in a standardized model of eye inflammation, endotoxin-induced uveitis (EIU). METHODS. Two hundred fifty nanograms of E. coli endotoxin was injected into the vitreous of knockout mIL-8Rh(-/-) (n = 7) mice or heterozygous littermate mIL-8Rh(+/-) controls (n = 7). Intravital microscopic examination of iris microvasculature was performed at baseline and 6 and 24 hours after endotoxin injection. The numbers of rolling (cells/mm2 endothelial surface/min), sticking (cells/mm2 endothelial surface), and infiltrating cells (cells/mm2 iris tissue) were evaluated by digital off-line quantification. RESULTS. The number of infiltrating cells was significantly reduced in mIL-8Rh(-/-) mice: 406 ± 77 cells/mm2 at 6 hours and 242 ± 50 cells/mm2 at 24 hours in mIL-8Rh(+/-) mice versus 14 ± 4 cells/mm2 at 6 hours and 38 ± 11 cells/mm2 at 24 hours in mIL-8Rh(-/-) mice (P <0.001). In contrast, the absence of the IL-8 receptor homologue did not reduce rolling or sticking. CONCLUSIONS. Iris rhodamine angiography allows precise quantification of leukocyte endothelial dynamics in the absence of surgical trauma. IL-8 and its homologues are known to be potent signals for leukocyte migration. Although IL-8 has previously been implicated in cell adhesion, video imaging in vivo demonstrated that deletion of the IL-8 receptor homologue had minimal effect on rolling or arrest in this model of inflammation.

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