Reduced apolipoprotein glycosylation in patients with the metabolic syndrome

Olga V. Savinova, Kristi Fillaus, Linhong Jing, William Harris, Gregory C. Shearer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: The purpose of this study was to compare the apolipoprotein composition of the three major lipoprotein classes in patients with metabolic syndrome to healthy controls. Methods: Very low density (VLDL), intermediate/low density (IDL/LDL, hereafter LDL), and high density lipoproteins (HDL) fractions were isolated from plasma of 56 metabolic syndrome subjects and from 14 age-sex matched healthy volunteers. The apolipoprotein content of fractions was analyzed by one-dimensional (1D) gel electrophoresis with confirmation by a combination of mass spectrometry and biochemical assays. Results: Metabolic syndrome patients differed from healthy controls in the following ways: (1) total plasma - apoA1 was lower, whereas apoB, apoC2, apoC3, and apoE were higher; (2) VLDL - apoB, apoC3, and apoE were increased; (3) LDL - apoC3 was increased, (4) HDL -associated constitutive serum amyloid A protein (SAA4) was reduced (p

Original languageEnglish (US)
Article numbere104833
JournalPLoS One
Volume9
Issue number8
DOIs
StatePublished - Aug 12 2014
Externally publishedYes

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Glycosylation
apolipoproteins
Apolipoproteins
metabolic syndrome
glycosylation
high density lipoprotein
Apolipoproteins B
Apolipoproteins E
HDL Lipoproteins
Apolipoprotein C-II
Plasmas
Serum Amyloid A Protein
amyloid
Electrophoresis
lipoproteins
gel electrophoresis
Lipoproteins
volunteers
Mass spectrometry
Assays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Savinova, O. V., Fillaus, K., Jing, L., Harris, W., & Shearer, G. C. (2014). Reduced apolipoprotein glycosylation in patients with the metabolic syndrome. PLoS One, 9(8), [e104833]. https://doi.org/10.1371/journal.pone.0104833

Reduced apolipoprotein glycosylation in patients with the metabolic syndrome. / Savinova, Olga V.; Fillaus, Kristi; Jing, Linhong; Harris, William; Shearer, Gregory C.

In: PLoS One, Vol. 9, No. 8, e104833, 12.08.2014.

Research output: Contribution to journalArticle

Savinova, OV, Fillaus, K, Jing, L, Harris, W & Shearer, GC 2014, 'Reduced apolipoprotein glycosylation in patients with the metabolic syndrome', PLoS One, vol. 9, no. 8, e104833. https://doi.org/10.1371/journal.pone.0104833
Savinova, Olga V. ; Fillaus, Kristi ; Jing, Linhong ; Harris, William ; Shearer, Gregory C. / Reduced apolipoprotein glycosylation in patients with the metabolic syndrome. In: PLoS One. 2014 ; Vol. 9, No. 8.
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