Redistribution of mu-opioid receptors in C1 adrenergic neurons following chronic administration of morphine

Carrie T. Drake, Sue Aicher, Fritzline L. Montalmant, Teresa A. Milner

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Neurons in the rostral ventrolateral medulla (RVLM) are involved in both tonic and reflex control of sympathetic outflow. Many of these neurons express the adrenaline-synthesizing enzyme phenylethanolamine N-methyltransferase (PNMT), and are designated C1 neurons. C1 neurons that contain mu-opioid receptors (MORs) are hyperpolarized by MOR activation and are activated during morphine withdrawal. The present study examined the subcellular distribution of the cloned MOR, MOR1, in rat C1 neurons following chronic morphine treatment, using RVLM sections that were dually labeled for PNMT-immunoperoxidase and MOR1-immunogold. Electron microscopic analysis of the subcellular distribution of MOR1 revealed a lower abundance of plasma membrane-associated MOR1 in C1 dendrites of rats treated with morphine, compared to placebo-treated controls, only in distal dendrites. There were no differences in the size of dual-labeled dendrites between treatment groups or in the overall density of MOR1 within PNMT immunoreactive dendrites between treatment groups. These results suggest that chronic morphine treatment leads to a decreased presence of MOR1 at the cell surface, without a significant reduction in cytoplasmic receptor density. These observations suggest that chronic morphine produces a selective internalization of MOR1 in C1 neurons, without apparent changes in receptor synthesis or trafficking. The reduction of accessible MORs on these neurons may be a mechanism for tolerance with regard to autonomic responses to opioid administration and may facilitate the profound sympathetic hyperactivity that occurs during acute opioid withdrawal.

Original languageEnglish (US)
Pages (from-to)365-372
Number of pages8
JournalExperimental Neurology
Volume196
Issue number2
DOIs
StatePublished - Dec 2005

Fingerprint

Adrenergic Neurons
mu Opioid Receptor
Morphine
Neurons
Phenylethanolamine N-Methyltransferase
Dendrites
Opioid Analgesics
Therapeutics
Cytoplasmic and Nuclear Receptors
Epinephrine
Reflex
Placebos
Cell Membrane
Electrons
Enzymes

Keywords

  • Electron microscopy
  • Receptor internalization
  • Rostral ventrolateral medulla

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Redistribution of mu-opioid receptors in C1 adrenergic neurons following chronic administration of morphine. / Drake, Carrie T.; Aicher, Sue; Montalmant, Fritzline L.; Milner, Teresa A.

In: Experimental Neurology, Vol. 196, No. 2, 12.2005, p. 365-372.

Research output: Contribution to journalArticle

Drake, Carrie T. ; Aicher, Sue ; Montalmant, Fritzline L. ; Milner, Teresa A. / Redistribution of mu-opioid receptors in C1 adrenergic neurons following chronic administration of morphine. In: Experimental Neurology. 2005 ; Vol. 196, No. 2. pp. 365-372.
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