Red blood cell-derived microparticles isolated from blood units initiate and propagate thrombin generation

Olivier Rubin, Julien Delobel, Michel Prudent, Niels Lion, Kid Kohl, Erik I. Tucker, Jean Daniel Tissot, Anne Angelillo-Scherrer

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Abstract

Background Red blood cell-derived microparticles (RMPs) are small phospholipid vesicles shed from RBCs in blood units, where they accumulate during storage. Because microparticles are bioactive, it could be suggested that RMPs are mediators of posttransfusion complications or, on the contrary, constitute a potential hemostatic agent. Study Design and Methods This study was performed to establish the impact on coagulation of RMPs isolated from blood units. Using calibrated automated thrombography, we investigated whether RMPs affect thrombin generation (TG) in plasma. Results We found that RMPs were not only able to increase TG in plasma in the presence of a low exogenous tissue factor (TF) concentration, but also to initiate TG in plasma in absence of exogenous TF. TG induced by RMPs in the absence of exogenous TF was neither affected by the presence of blocking anti-TF nor by the absence of Factor (F)VII. It was significantly reduced in plasma deficient in FVIII or F IX and abolished in FII-, FV-, FX-, or FXI-deficient plasma. TG was also totally abolished when anti-XI 01A6 was added in the sample. Finally, neither Western blotting, flow cytometry, nor immunogold labeling allowed the detection of traces of TF antigen. In addition, RMPs did not comprise polyphosphate, an important modulator of coagulation. Conclusions Taken together, our data show that RMPs have FXI-dependent procoagulant properties and are able to initiate and propagate TG. The anionic surface of RMPs might be the site of FXI-mediated TG amplification and intrinsic tenase and prothrombinase complex assembly.

Original languageEnglish (US)
Pages (from-to)1744-1754
Number of pages11
JournalTransfusion
Volume53
Issue number8
DOIs
StatePublished - Aug 1 2013

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Rubin, O., Delobel, J., Prudent, M., Lion, N., Kohl, K., Tucker, E. I., Tissot, J. D., & Angelillo-Scherrer, A. (2013). Red blood cell-derived microparticles isolated from blood units initiate and propagate thrombin generation. Transfusion, 53(8), 1744-1754. https://doi.org/10.1111/trf.12008