Recruitment of TRADD, FADD, and caspase 8 to double-stranded RNA-triggered death inducing signaling complexes (dsRNA-DISCs)

M. S. Iordanov, J. D. Kirsch, O. P. Ryabinina, J. Wong, P. N. Spitz, Veselina Korcheva, A. Thorburn, B. E. Magun

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Rapid elimination of virus-infected cells by apoptosis is an efficient anti-viral strategy. Double-stranded RNA (dsRNA), a viral product, is potently and rapidly apoptogenic in susceptible cells. Caspase 8 plays an important role in the dsRNA-induced apoptosis; however, the mechanisms of caspase 8 activation in response to dsRNA are unknown. We demonstrate here that, in HeLa cells, the dsRNA-triggered activation of caspase 8 is independent of ongoing proteins synthesis (and is, therefore, independent of changes in pro- and anti-apoptotic gene expression) and involves the formation of multiprotein dsRNA-triggered death inducing signaling complexes (dsRNA-DISCs). DsRNA-DISCs contain FADD, TRADD, and caspase 8; however, several experimental approaches suggest that death ligands and death receptors (such as Fas/Apo1 and DR4/Apo2) are not involved in the formation of dsRNA-DISCs.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalApoptosis
Volume10
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

Death Domain Receptor Signaling Adaptor Proteins
Double-Stranded RNA
Caspase 8
Chemical activation
Apoptosis
Death Domain Receptors
Viruses
HeLa Cells
Gene expression
Ligands
Gene Expression

Keywords

  • Apoptosis
  • Caspases
  • Death receptor
  • DISC
  • Double-stranded
  • FADD
  • TRADD

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Recruitment of TRADD, FADD, and caspase 8 to double-stranded RNA-triggered death inducing signaling complexes (dsRNA-DISCs). / Iordanov, M. S.; Kirsch, J. D.; Ryabinina, O. P.; Wong, J.; Spitz, P. N.; Korcheva, Veselina; Thorburn, A.; Magun, B. E.

In: Apoptosis, Vol. 10, No. 1, 01.2005, p. 167-176.

Research output: Contribution to journalArticle

Iordanov, MS, Kirsch, JD, Ryabinina, OP, Wong, J, Spitz, PN, Korcheva, V, Thorburn, A & Magun, BE 2005, 'Recruitment of TRADD, FADD, and caspase 8 to double-stranded RNA-triggered death inducing signaling complexes (dsRNA-DISCs)', Apoptosis, vol. 10, no. 1, pp. 167-176. https://doi.org/10.1007/s10495-005-6071-x
Iordanov, M. S. ; Kirsch, J. D. ; Ryabinina, O. P. ; Wong, J. ; Spitz, P. N. ; Korcheva, Veselina ; Thorburn, A. ; Magun, B. E. / Recruitment of TRADD, FADD, and caspase 8 to double-stranded RNA-triggered death inducing signaling complexes (dsRNA-DISCs). In: Apoptosis. 2005 ; Vol. 10, No. 1. pp. 167-176.
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