Recruitment of TRADD, FADD, and caspase 8 to double-stranded RNA-triggered death inducing signaling complexes (dsRNA-DISCs)

M. S. Iordanov, J. D. Kirsch, O. P. Ryabinina, J. Wong, P. N. Spitz, V. B. Korcheva, A. Thorburn, B. E. Magun

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Rapid elimination of virus-infected cells by apoptosis is an efficient anti-viral strategy. Double-stranded RNA (dsRNA), a viral product, is potently and rapidly apoptogenic in susceptible cells. Caspase 8 plays an important role in the dsRNA-induced apoptosis; however, the mechanisms of caspase 8 activation in response to dsRNA are unknown. We demonstrate here that, in HeLa cells, the dsRNA-triggered activation of caspase 8 is independent of ongoing proteins synthesis (and is, therefore, independent of changes in pro- and anti-apoptotic gene expression) and involves the formation of multiprotein dsRNA-triggered death inducing signaling complexes (dsRNA-DISCs). DsRNA-DISCs contain FADD, TRADD, and caspase 8; however, several experimental approaches suggest that death ligands and death receptors (such as Fas/Apo1 and DR4/Apo2) are not involved in the formation of dsRNA-DISCs.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalApoptosis
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2005

Keywords

  • Apoptosis
  • Caspases
  • DISC
  • Death receptor
  • Double-stranded
  • FADD
  • TRADD

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Cancer Research

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