Recruitment of CREB binding protein is sufficient for CREB-mediated gene activation

Jean Rene Cardinaux, John C. Notis, Qinghong Zhang, Ngan Vo, Johanna C. Craig, Daniel M. Fass, Richard G. Brennan, Richard H. Goodman

Research output: Contribution to journalArticle

143 Scopus citations

Abstract

Phosphorylation of the transcription factor CREB leads to the recruitment of the coactivator, CREB binding protein (CBP). Recent studies have suggested that CBP recruitment is not sufficient for CREB function, however. We have identified a conserved protein-protein interaction motif within the CBP-binding domains of CREB and another transcription factor, SREBP (sterol-responsive element binding protein). In contrast to CREB, SREBP interacts with CBP in the absence of phosphorylation. We have exploited the conservation of this interaction motif to test whether CBP recruitment to CREB is sufficient for transcriptional activation. Substitution of six nonconserved amino acids from SREBP into the activation domain of CREB confers high-affinity, phosphorylation-independent CBP binding. The mutated CREB molecule, CREB(DIEDML), activates transcription in F9 teratocarcinoma and PC12 cells even in the absence of protein kinase A (PKA). Addition of exogenous CBP augments the level of transcription mediated by CREB(DIEDML), and adenovirus 12S E1A blocks transcription, implicating CBP in the activation process. Thus, recruitment of CBP to CREB is sufficient for transcriptional activation. Addition of PKA stimulates transcription induced by CREB(DIEDML) further, suggesting that a phosphorylation event downstream from CBP recruitment augments CREB signaling.

Original languageEnglish (US)
Pages (from-to)1546-1552
Number of pages7
JournalMolecular and cellular biology
Volume20
Issue number5
DOIs
StatePublished - Mar 2000

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Cardinaux, J. R., Notis, J. C., Zhang, Q., Vo, N., Craig, J. C., Fass, D. M., Brennan, R. G., & Goodman, R. H. (2000). Recruitment of CREB binding protein is sufficient for CREB-mediated gene activation. Molecular and cellular biology, 20(5), 1546-1552. https://doi.org/10.1128/MCB.20.5.1546-1552.2000