Recovery of Drug Delivery Nanoparticles from Human Plasma Using an Electrokinetic Platform Technology

Stuart Ibsen, Avery Sonnenberg, Carolyn Schutt, Rajesh Mukthavaram, Yasan Yeh, Inanc Ortac, Sareh Manouchehri, Santosh Kesari, Sadik Esener, Michael (Mike) Heller

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of complex biological fluids on the surface and structure of nanoparticles is a rapidly expanding field of study. One of the challenges holding back this research is the difficulty of recovering therapeutic nanoparticles from biological samples due to their small size, low density, and stealth surface coatings. Here, the first demonstration of the recovery and analysis of drug delivery nanoparticles from undiluted human plasma samples through the use of a new electrokinetic platform technology is presented. The particles are recovered from plasma through a dielectrophoresis separation force that is created by innate differences in the dielectric properties between the unaltered nanoparticles and the surrounding plasma. It is shown that this can be applied to a wide range of drug delivery nanoparticles of different morphologies and materials, including low-density nanoliposomes. These recovered particles can then be analyzed using different methods including scanning electron microscopy to monitor surface and structural changes that result from plasma exposure. This new recovery technique can be broadly applied to the recovery of nanoparticles from high conductance fluids in a wide range of applications. Drug delivery nanoparticles are recovered from undiluted plasma through the creation of a dielectrophoresis separation force. This force is based on innate differences in the dielectric properties between the nanoparticles and the plasma. Nanoparticles in plasma are pulled down to electrodes using an alternating electric field. A wash then removes the bulk plasma and the purified nanoparticles are recovered.

Original languageEnglish (US)
Pages (from-to)5088-5096
Number of pages9
JournalSmall
Volume11
Issue number38
DOIs
StatePublished - Oct 1 2015
Externally publishedYes

Fingerprint

Plasma (human)
Drug delivery
Nanoparticles
Technology
Recovery
Pharmaceutical Preparations
Plasmas
Electrophoresis
Dielectric properties
Fluids
Electron Scanning Microscopy
Electrodes
Demonstrations
Electric fields

Keywords

  • dielectrophoresis
  • drug delivery nanoparticles
  • nanoparticle surfaces
  • plasma
  • recovery

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology

Cite this

Recovery of Drug Delivery Nanoparticles from Human Plasma Using an Electrokinetic Platform Technology. / Ibsen, Stuart; Sonnenberg, Avery; Schutt, Carolyn; Mukthavaram, Rajesh; Yeh, Yasan; Ortac, Inanc; Manouchehri, Sareh; Kesari, Santosh; Esener, Sadik; Heller, Michael (Mike).

In: Small, Vol. 11, No. 38, 01.10.2015, p. 5088-5096.

Research output: Contribution to journalArticle

Ibsen, S, Sonnenberg, A, Schutt, C, Mukthavaram, R, Yeh, Y, Ortac, I, Manouchehri, S, Kesari, S, Esener, S & Heller, MM 2015, 'Recovery of Drug Delivery Nanoparticles from Human Plasma Using an Electrokinetic Platform Technology', Small, vol. 11, no. 38, pp. 5088-5096. https://doi.org/10.1002/smll.201500892
Ibsen, Stuart ; Sonnenberg, Avery ; Schutt, Carolyn ; Mukthavaram, Rajesh ; Yeh, Yasan ; Ortac, Inanc ; Manouchehri, Sareh ; Kesari, Santosh ; Esener, Sadik ; Heller, Michael (Mike). / Recovery of Drug Delivery Nanoparticles from Human Plasma Using an Electrokinetic Platform Technology. In: Small. 2015 ; Vol. 11, No. 38. pp. 5088-5096.
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