Recordings from Single Neocortical Nerve Terminals Reveal a Nonselective Cation Channel Activated by Decreases in Extracellular Calcium

Stephen Smith, Jeremy B. Bergsman, Nobutoshi C. Harata, Richard H. Scheller, Richard W. Tsien

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Synaptic activity causes reductions in cleft [Ca2+] that may impact subsequent synaptic efficacy. Using modified patch-clamp techniques to record from single neocortical nerve terminals, we report that physiologically relevant reductions of extracellular [Ca2+] ([Ca2+] o) activate voltage-dependent outward currents. These outward currents are carried by a novel nonselective cation (NSC) channel that is indirectly inhibited by various extracellular agents (rank order potency, Gd3+ > spermidine > Ca2+ > Mg2+, typical for [Ca2+]o receptors). The identification of a Ca2+ sensor-NSC channel pathway establishes the existence of a mechanism by which presynaptic terminals can detect and respond to reductions in cleft [Ca2+]. Activation of NSC channels by falls in [Ca 2+]o would be expected during periods of high activity in the neocortex and may modulate the excitability of the presynaptic terminal.

Original languageEnglish (US)
Pages (from-to)243-256
Number of pages14
JournalNeuron
Volume41
Issue number2
DOIs
StatePublished - Jan 22 2004

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Cations
Presynaptic Terminals
Calcium
Spermidine
Neocortex
Patch-Clamp Techniques

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Recordings from Single Neocortical Nerve Terminals Reveal a Nonselective Cation Channel Activated by Decreases in Extracellular Calcium. / Smith, Stephen; Bergsman, Jeremy B.; Harata, Nobutoshi C.; Scheller, Richard H.; Tsien, Richard W.

In: Neuron, Vol. 41, No. 2, 22.01.2004, p. 243-256.

Research output: Contribution to journalArticle

Smith, Stephen ; Bergsman, Jeremy B. ; Harata, Nobutoshi C. ; Scheller, Richard H. ; Tsien, Richard W. / Recordings from Single Neocortical Nerve Terminals Reveal a Nonselective Cation Channel Activated by Decreases in Extracellular Calcium. In: Neuron. 2004 ; Vol. 41, No. 2. pp. 243-256.
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