Reconstitution of interactions of Murine gammaherpesvirus 68 M11 with Bcl-2 family proteins in yeast

Barbora Juhásová, Ingrid Bhatia-Kiššová, Katarína Polčicová, Marek Mentel, Michael Forte, Peter Polčic

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

One of the mechanisms of defense against viral infection is induction of apoptosis in infected cells. To escape this line of protection, genomes of many viruses encode for proteins that inhibit apoptosis. Murid herpesvirus 4 gene M11 encodes for homologue of cellular Bcl-2 proteins that inhibits apoptosis and autophagy in infected cell. To study a role of M11 in regulation of apoptosis we have established a yeast model system in which the action of M11 together with proapoptotic proteins Bax, Bak and Bid can be studied. When expressed in yeast, M11 did not inhibit autophagic pathway, so only effects of expression of M11 on activity of coexpressed proapoptotic proteins could be observed. In this experimental setting M11 potently inhibited both proapoptotic multidomain proteins Bax and Bak. The antiapoptotic activity of M11 was suppressed by coexpression of proapoptotic BH3-only protein tBid, indicating that M11 inhibits apoptosis likely by the same mechanism as cellular antiapoptotic proteins Bcl-2 or Bcl-XL.

Original languageEnglish (US)
Pages (from-to)783-787
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume407
Issue number4
DOIs
StatePublished - Apr 22 2011

Fingerprint

Fungal Proteins
Yeast
Apoptosis
BH3 Interacting Domain Death Agonist Protein
bcl-2 Homologous Antagonist-Killer Protein
bcl-2-Associated X Protein
Proteins
Rhadinovirus
Genes
Yeasts
Autophagy
Virus Diseases
Viruses
Genome

Keywords

  • Apoptosis
  • Bak
  • Bax
  • M11
  • Murid herpesvirus 4
  • TBid
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Reconstitution of interactions of Murine gammaherpesvirus 68 M11 with Bcl-2 family proteins in yeast. / Juhásová, Barbora; Bhatia-Kiššová, Ingrid; Polčicová, Katarína; Mentel, Marek; Forte, Michael; Polčic, Peter.

In: Biochemical and Biophysical Research Communications, Vol. 407, No. 4, 22.04.2011, p. 783-787.

Research output: Contribution to journalArticle

Juhásová, Barbora ; Bhatia-Kiššová, Ingrid ; Polčicová, Katarína ; Mentel, Marek ; Forte, Michael ; Polčic, Peter. / Reconstitution of interactions of Murine gammaherpesvirus 68 M11 with Bcl-2 family proteins in yeast. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 407, No. 4. pp. 783-787.
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