Recombination‐deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages

Nancy A. Buchmeier, Craig J. Lipps, Magdalene Y.H. So, Fred Heffron

    Research output: Contribution to journalArticle

    136 Scopus citations

    Abstract

    Mutations in the genes recA and recBC were constructed in the virulent Salmonella typhimurium strain 14028s. Both the recA and recBC mutants were attenuated in mice. The mutants were also sensitive to killing by macrophages in vitro. The recombination mutants were no longer macrophage sensitive in a variant line of J774 macrophage‐like cells that fail to generate superoxide. This suggests that repair of DNA damage by Salmonella is necessary for full virulence in vivo and that the oxidative burst of phagocytes is one source of such DNA damage.

    Original languageEnglish (US)
    Pages (from-to)933-936
    Number of pages4
    JournalMolecular Microbiology
    Volume7
    Issue number6
    DOIs
    StatePublished - Mar 1993

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology

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