Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques

K. K. Steger; P. J. Valentine; F. Heffron; M. So; C. D. Pauza

doi:10.1016/S0264-410X(98)00278-3

Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques

K. K. Steger, P. J. Valentine, F. Heffron, M. So, C. D. Pauza

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Recombinant bacteria are useful vectors for delivering foreign antigens to muscosal surfaces and may elicit immune protection against sexually- transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.

Original language	English (US)
Pages (from-to)	923-932
Number of pages	10
Journal	Vaccine
Volume	17
Issue number	7-8
DOIs	https://doi.org/10.1016/S0264-410X(98)00278-3
State	Published - Feb 26 1999
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/S0264-410X(98)00278-3

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title = "Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques",

abstract = "Recombinant bacteria are useful vectors for delivering foreign antigens to muscosal surfaces and may elicit immune protection against sexually- transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.",

author = "Steger, {K. K.} and Valentine, {P. J.} and F. Heffron and M. So and Pauza, {C. D.}",

note = "Funding Information: This work was supported by PHS Grants AI33237 and AI36643 (CDP) and RR00167 (Regional Primate Research Center support). This publication is number 38-007 from the Wisconsin Regional Primate Research Center.",

year = "1999",

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doi = "10.1016/S0264-410X(98)00278-3",

language = "English (US)",

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AU - Steger, K. K.

AU - Valentine, P. J.

AU - Heffron, F.

AU - So, M.

AU - Pauza, C. D.

N1 - Funding Information: This work was supported by PHS Grants AI33237 and AI36643 (CDP) and RR00167 (Regional Primate Research Center support). This publication is number 38-007 from the Wisconsin Regional Primate Research Center.

PY - 1999/2/26

Y1 - 1999/2/26

N2 - Recombinant bacteria are useful vectors for delivering foreign antigens to muscosal surfaces and may elicit immune protection against sexually- transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.

AB - Recombinant bacteria are useful vectors for delivering foreign antigens to muscosal surfaces and may elicit immune protection against sexually- transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.

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Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques

Abstract

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