Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques

K. K. Steger, P. J. Valentine, Fred Heffron, M. So, C. D. Pauza

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Recombinant bacteria are useful vectors for delivering foreign antigens to muscosal surfaces and may elicit immune protection against sexually- transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.

Original languageEnglish (US)
Pages (from-to)923-932
Number of pages10
JournalVaccine
Volume17
Issue number7-8
DOIs
StatePublished - Feb 26 1999
Externally publishedYes

Fingerprint

capsid
Capsid
Salmonella typhimurium
Macaca mulatta
Salmonella Typhimurium
Immunization
immunization
antigens
Antigens
Intramuscular Injections
intramuscular injection
blood serum
Salmonella
Simian immunodeficiency virus
Secondary Immunization
Simian Immunodeficiency Virus
antibodies
Capsid Proteins
Macaca
coat proteins

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques. / Steger, K. K.; Valentine, P. J.; Heffron, Fred; So, M.; Pauza, C. D.

In: Vaccine, Vol. 17, No. 7-8, 26.02.1999, p. 923-932.

Research output: Contribution to journalArticle

Steger, K. K. ; Valentine, P. J. ; Heffron, Fred ; So, M. ; Pauza, C. D. / Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques. In: Vaccine. 1999 ; Vol. 17, No. 7-8. pp. 923-932.
@article{486be38b428d4b3ab993a656f485b426,
title = "Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques",
abstract = "Recombinant bacteria are useful vectors for delivering foreign antigens to muscosal surfaces and may elicit immune protection against sexually- transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.",
author = "Steger, {K. K.} and Valentine, {P. J.} and Fred Heffron and M. So and Pauza, {C. D.}",
year = "1999",
month = "2",
day = "26",
doi = "10.1016/S0264-410X(98)00278-3",
language = "English (US)",
volume = "17",
pages = "923--932",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "7-8",

}

TY - JOUR

T1 - Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques

AU - Steger, K. K.

AU - Valentine, P. J.

AU - Heffron, Fred

AU - So, M.

AU - Pauza, C. D.

PY - 1999/2/26

Y1 - 1999/2/26

N2 - Recombinant bacteria are useful vectors for delivering foreign antigens to muscosal surfaces and may elicit immune protection against sexually- transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.

AB - Recombinant bacteria are useful vectors for delivering foreign antigens to muscosal surfaces and may elicit immune protection against sexually- transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.

UR - http://www.scopus.com/inward/record.url?scp=0033605375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033605375&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(98)00278-3

DO - 10.1016/S0264-410X(98)00278-3

M3 - Article

C2 - 10067699

AN - SCOPUS:0033605375

VL - 17

SP - 923

EP - 932

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 7-8

ER -