Receptor expression in mammalian cells

Rachael L. Neve, Kim A. Neve

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The methods outlined above for the cloning of dopamine receptor cDNAs and their expression in mammalian cells allow the investigator to study dopamine receptor pharmacology with a precision and detail not previously possible. These strategies can be used to create stable and clonal genetically engineered cell lines that express a given receptor subtype, a specific spliced form of a receptor subtype, or mutant and chimeric receptors. Such transfected cells can be evaluated to determine how receptors bind ligands in a receptor-selective manner, to learn how receptors transduce the energy of the binding of agonists into a functional response, to ascertain the mechanisms by which specificity or selectivity of coupling of receptors to various G proteins and signaling pathways is attained, and to reveal the molecular basis of regulation of receptor sensitivity.

Original languageEnglish (US)
Pages (from-to)163-174
Number of pages12
JournalMethods in Neurosciences
Volume25
Issue numberC
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Neuroscience(all)

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