The methods outlined above for the cloning of dopamine receptor cDNAs and their expression in mammalian cells allow the investigator to study dopamine receptor pharmacology with a precision and detail not previously possible. These strategies can be used to create stable and clonal genetically engineered cell lines that express a given receptor subtype, a specific spliced form of a receptor subtype, or mutant and chimeric receptors. Such transfected cells can be evaluated to determine how receptors bind ligands in a receptor-selective manner, to learn how receptors transduce the energy of the binding of agonists into a functional response, to ascertain the mechanisms by which specificity or selectivity of coupling of receptors to various G proteins and signaling pathways is attained, and to reveal the molecular basis of regulation of receptor sensitivity.
|Original language||English (US)|
|Number of pages||12|
|Journal||Methods in Neurosciences|
|State||Published - Jan 1 1995|
ASJC Scopus subject areas