Receptive field properties of starburst cholinergic amacrine cells in the rabbit retina

William Taylor, H. Wassle

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Patch-clamp recordings were made from ON starburst cholinergic amacrine cells with somas located in the ganglion cell layer of an isolated, dark-adapted rabbit retina preparation. Light responses were analysed and cell identity was confirmed anatomically. The centre light response had a linear current-voltage relation with a reversal potential close to 0 mV. The receptive field size was similar to the dendritic field size. Cholinergic amacrine cells displayed significant surround inhibition. The receptive field profile consisted of a central excitatory region flanked by an inhibitory surround. The surround attenuated the central response to 36% of the maximum. The surround was probably mediated by a combination of presynaptic and postsynaptic inhibition. Starburst amacrine cells did not display action potentials and the presence of a large, voltage-dependent outward current limited depolarizing responses to a maximum potential of about -40 mV. Light responses were completely suppressed during application of 100 μM D,L-2-amino-4-phosphonobutyric acid (APE), consistent with activation exclusively through rod bipolar cells (on) and ON-cone bipolar cells. In darkness the cells displayed a tonic inward current that could be blocked by 100 μM APE and 2 μM CNQX.

Original languageEnglish (US)
Pages (from-to)2308-2321
Number of pages14
JournalEuropean Journal of Neuroscience
Volume7
Issue number11
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Amacrine Cells
Cholinergic Agents
Retina
Rabbits
Light
6-Cyano-7-nitroquinoxaline-2,3-dione
Darkness
Carisoprodol
Ganglia
Action Potentials

Keywords

  • Direction sensitivity
  • Electrophysiology
  • Light response
  • Voltage clamp

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Receptive field properties of starburst cholinergic amacrine cells in the rabbit retina. / Taylor, William; Wassle, H.

In: European Journal of Neuroscience, Vol. 7, No. 11, 1995, p. 2308-2321.

Research output: Contribution to journalArticle

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