Real time three-dimensional echocardiographic evaluations of fetal left ventricular stroke volume, mass, and myocardial strain: In vitro and in vivo experimental study

Meihua Zhu, Muhammad Ashraf, Zhijun Zhang, Cole Streiff, Eriko Shimada, Sumito Kimura, Traci Schaller, Xubo Song, David Sahn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background Left ventricular stroke volume, mass, and myocardial strain are valuable indicators of fetal heart function. This study investigated the feasibility of nongated real time three-dimensional echocardiography (RT3DE) to determine fetal stroke volume (SV), left ventricular mass (LVM), and myocardial strain under different conditions. Methods To evaluate fetal hearts, fetal-sized rabbit hearts were used in this study. The in vitro portion of this study was carried out using a balloon inserted into the LV of eight fresh rabbit hearts and driven by a calibrated pulsatile pump. RT3DE volumes were obtained at various pump-set SVs. The in vivo experiments in this study were performed on open-chest rabbits. RT3DE volumes were acquired at the following conditions: baseline, simulated hypervolemia, inferior vena cava (IVC) ligation, and ascending aorta (AAO) ligation. Displacement values and sonomicrometry data were used as references for RT3DE-derived SV, LVM, longitudinal strain (LS), and circumferential strain (CS). Results Excellent correlations between RT3DE-derived values and reference values were demonstrated and accompanied by high coefficients of determination (R2) for both in vitro and in vivo studies for SV, LVM, LS, and CS (in vitro: SV: R2 = 0.98; LVM: R2 = 0.97; LS: R2 = 0.87, CS: R2 = 0.80; in vivo: SV: R2 = 0.92; LVM: R2 = 0.98; LS: in vivo: R2 = 0.84; CS: in vivo: R2 = 0.76; all P <0.05). Conclusions RT3DE is capable of quantifying the SV, LVM, and myocardial strain of fetal-sized hearts under different conditions. This nongated RT3DE may aid the evaluation of fetal cardiac function, providing a superior understanding of the progress of fetal heart disorders.

Original languageEnglish (US)
Pages (from-to)1697-1706
Number of pages10
JournalEchocardiography
Volume32
Issue number11
DOIs
StatePublished - Nov 1 2015

Fingerprint

Stroke Volume
Fetal Heart
Rabbits
Ligation
Three-Dimensional Echocardiography
Inferior Vena Cava
Feasibility Studies
In Vitro Techniques
Aorta
Reference Values
Thorax

Keywords

  • fetal echocardiography
  • left ventricular mass, strain
  • real time imaging technique

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Real time three-dimensional echocardiographic evaluations of fetal left ventricular stroke volume, mass, and myocardial strain : In vitro and in vivo experimental study. / Zhu, Meihua; Ashraf, Muhammad; Zhang, Zhijun; Streiff, Cole; Shimada, Eriko; Kimura, Sumito; Schaller, Traci; Song, Xubo; Sahn, David.

In: Echocardiography, Vol. 32, No. 11, 01.11.2015, p. 1697-1706.

Research output: Contribution to journalArticle

Zhu, Meihua ; Ashraf, Muhammad ; Zhang, Zhijun ; Streiff, Cole ; Shimada, Eriko ; Kimura, Sumito ; Schaller, Traci ; Song, Xubo ; Sahn, David. / Real time three-dimensional echocardiographic evaluations of fetal left ventricular stroke volume, mass, and myocardial strain : In vitro and in vivo experimental study. In: Echocardiography. 2015 ; Vol. 32, No. 11. pp. 1697-1706.
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abstract = "Background Left ventricular stroke volume, mass, and myocardial strain are valuable indicators of fetal heart function. This study investigated the feasibility of nongated real time three-dimensional echocardiography (RT3DE) to determine fetal stroke volume (SV), left ventricular mass (LVM), and myocardial strain under different conditions. Methods To evaluate fetal hearts, fetal-sized rabbit hearts were used in this study. The in vitro portion of this study was carried out using a balloon inserted into the LV of eight fresh rabbit hearts and driven by a calibrated pulsatile pump. RT3DE volumes were obtained at various pump-set SVs. The in vivo experiments in this study were performed on open-chest rabbits. RT3DE volumes were acquired at the following conditions: baseline, simulated hypervolemia, inferior vena cava (IVC) ligation, and ascending aorta (AAO) ligation. Displacement values and sonomicrometry data were used as references for RT3DE-derived SV, LVM, longitudinal strain (LS), and circumferential strain (CS). Results Excellent correlations between RT3DE-derived values and reference values were demonstrated and accompanied by high coefficients of determination (R2) for both in vitro and in vivo studies for SV, LVM, LS, and CS (in vitro: SV: R2 = 0.98; LVM: R2 = 0.97; LS: R2 = 0.87, CS: R2 = 0.80; in vivo: SV: R2 = 0.92; LVM: R2 = 0.98; LS: in vivo: R2 = 0.84; CS: in vivo: R2 = 0.76; all P <0.05). Conclusions RT3DE is capable of quantifying the SV, LVM, and myocardial strain of fetal-sized hearts under different conditions. This nongated RT3DE may aid the evaluation of fetal cardiac function, providing a superior understanding of the progress of fetal heart disorders.",
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T1 - Real time three-dimensional echocardiographic evaluations of fetal left ventricular stroke volume, mass, and myocardial strain

T2 - In vitro and in vivo experimental study

AU - Zhu, Meihua

AU - Ashraf, Muhammad

AU - Zhang, Zhijun

AU - Streiff, Cole

AU - Shimada, Eriko

AU - Kimura, Sumito

AU - Schaller, Traci

AU - Song, Xubo

AU - Sahn, David

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Background Left ventricular stroke volume, mass, and myocardial strain are valuable indicators of fetal heart function. This study investigated the feasibility of nongated real time three-dimensional echocardiography (RT3DE) to determine fetal stroke volume (SV), left ventricular mass (LVM), and myocardial strain under different conditions. Methods To evaluate fetal hearts, fetal-sized rabbit hearts were used in this study. The in vitro portion of this study was carried out using a balloon inserted into the LV of eight fresh rabbit hearts and driven by a calibrated pulsatile pump. RT3DE volumes were obtained at various pump-set SVs. The in vivo experiments in this study were performed on open-chest rabbits. RT3DE volumes were acquired at the following conditions: baseline, simulated hypervolemia, inferior vena cava (IVC) ligation, and ascending aorta (AAO) ligation. Displacement values and sonomicrometry data were used as references for RT3DE-derived SV, LVM, longitudinal strain (LS), and circumferential strain (CS). Results Excellent correlations between RT3DE-derived values and reference values were demonstrated and accompanied by high coefficients of determination (R2) for both in vitro and in vivo studies for SV, LVM, LS, and CS (in vitro: SV: R2 = 0.98; LVM: R2 = 0.97; LS: R2 = 0.87, CS: R2 = 0.80; in vivo: SV: R2 = 0.92; LVM: R2 = 0.98; LS: in vivo: R2 = 0.84; CS: in vivo: R2 = 0.76; all P <0.05). Conclusions RT3DE is capable of quantifying the SV, LVM, and myocardial strain of fetal-sized hearts under different conditions. This nongated RT3DE may aid the evaluation of fetal cardiac function, providing a superior understanding of the progress of fetal heart disorders.

AB - Background Left ventricular stroke volume, mass, and myocardial strain are valuable indicators of fetal heart function. This study investigated the feasibility of nongated real time three-dimensional echocardiography (RT3DE) to determine fetal stroke volume (SV), left ventricular mass (LVM), and myocardial strain under different conditions. Methods To evaluate fetal hearts, fetal-sized rabbit hearts were used in this study. The in vitro portion of this study was carried out using a balloon inserted into the LV of eight fresh rabbit hearts and driven by a calibrated pulsatile pump. RT3DE volumes were obtained at various pump-set SVs. The in vivo experiments in this study were performed on open-chest rabbits. RT3DE volumes were acquired at the following conditions: baseline, simulated hypervolemia, inferior vena cava (IVC) ligation, and ascending aorta (AAO) ligation. Displacement values and sonomicrometry data were used as references for RT3DE-derived SV, LVM, longitudinal strain (LS), and circumferential strain (CS). Results Excellent correlations between RT3DE-derived values and reference values were demonstrated and accompanied by high coefficients of determination (R2) for both in vitro and in vivo studies for SV, LVM, LS, and CS (in vitro: SV: R2 = 0.98; LVM: R2 = 0.97; LS: R2 = 0.87, CS: R2 = 0.80; in vivo: SV: R2 = 0.92; LVM: R2 = 0.98; LS: in vivo: R2 = 0.84; CS: in vivo: R2 = 0.76; all P <0.05). Conclusions RT3DE is capable of quantifying the SV, LVM, and myocardial strain of fetal-sized hearts under different conditions. This nongated RT3DE may aid the evaluation of fetal cardiac function, providing a superior understanding of the progress of fetal heart disorders.

KW - fetal echocardiography

KW - left ventricular mass, strain

KW - real time imaging technique

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