Real-time quantitative polymerase chain reaction and culture analyses of Enterococcus faecalis in root canals

Christine Sedgley, Aaron Nagel, Gunnar Dahlén, Claes Reit, Anders Molander

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal sample. Consecutive root canal samples obtained from primary infection (n = 40) and retreatment (n = 48) cases were divided into two equal aliquots that were independently analyzed using culture and qPCR by investigators blinded to the analysis results of the other sample. E. faecalis was detected in 10.2% and 79.5% of samples by culture and qPCR, respectively (p < 0.0001; McNemar's test). E. faecalis was detected in more retreatment than primary infection samples (89.6% versus 67.5%; p = 0.01, Fisher's exact test). qPCR reported a significantly higher prevalence of E. faecalis in endodontic samples than culture techniques.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalJournal of endodontics
Volume32
Issue number3
DOIs
StatePublished - Mar 1 2006

Keywords

  • Culture
  • Endodontic
  • Enterococci
  • Enterococcus faecalis
  • Real-time quantitative PCR

ASJC Scopus subject areas

  • Dentistry(all)

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