TY - JOUR
T1 - Real-time imaging and quantification of bioluminescent bacteria in root canals in vitro
AU - Sedgley, Christine
AU - Applegate, Bruce
AU - Nagel, Aaron
AU - Hall, Dan
PY - 2004/12
Y1 - 2004/12
N2 - The detection of microorganisms in root canals is generally limited to qualitative or semiquantitative methods. We describe a new and nondestructive in vitro method to quantify root-canal bacteria over sequential treatment procedures using real-time imaging in conjunction with the bioluminescent reporter strain Pseudomonas fluorescens 5RL. Induced bacterial photon emission can be monitored by sensitive optical photonic imaging and luminometry, providing images as well as spatial and quantitative measurements. Bioluminescence imaging and luminometry determined that the lower limit of detection of bacteria in root canals occurred between 2 × 102 and 2 × 103 cells, with high correlation between cell counts and detection devices (r ≥ 0.981). A preliminary study assessed the efficacy of sequential irrigation procedures to remove 5 × 106 bacteria from root canals (n = 5; apical size 60) using a 28-gauge, endodontic needle positioned 1 mm from working length; 9.2% ± 3.1% and 8% ± 3.6% of bacteria remained after 3 and 6 ml irrigation, respectively (p = 0.03), corresponding to approximately 4 × 105 bacteria remaining after 6 ml. This method can be used to study the efficacy of sequential endodontic treatment procedures in removing bacteria from root canals.
AB - The detection of microorganisms in root canals is generally limited to qualitative or semiquantitative methods. We describe a new and nondestructive in vitro method to quantify root-canal bacteria over sequential treatment procedures using real-time imaging in conjunction with the bioluminescent reporter strain Pseudomonas fluorescens 5RL. Induced bacterial photon emission can be monitored by sensitive optical photonic imaging and luminometry, providing images as well as spatial and quantitative measurements. Bioluminescence imaging and luminometry determined that the lower limit of detection of bacteria in root canals occurred between 2 × 102 and 2 × 103 cells, with high correlation between cell counts and detection devices (r ≥ 0.981). A preliminary study assessed the efficacy of sequential irrigation procedures to remove 5 × 106 bacteria from root canals (n = 5; apical size 60) using a 28-gauge, endodontic needle positioned 1 mm from working length; 9.2% ± 3.1% and 8% ± 3.6% of bacteria remained after 3 and 6 ml irrigation, respectively (p = 0.03), corresponding to approximately 4 × 105 bacteria remaining after 6 ml. This method can be used to study the efficacy of sequential endodontic treatment procedures in removing bacteria from root canals.
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U2 - 10.1097/01.DON.0000132299.02265.6C
DO - 10.1097/01.DON.0000132299.02265.6C
M3 - Article
C2 - 15564873
AN - SCOPUS:10044235690
SN - 0099-2399
VL - 30
SP - 893
EP - 898
JO - Journal of endodontics
JF - Journal of endodontics
IS - 12
ER -