Reactivation of latent human cytomegalovirus by allogeneic stimulation of blood cells from healthy donors

Cecilia Söderberg-Nauclér, Kenneth N. Fish, Jay A. Nelson

Research output: Contribution to journalArticle

514 Scopus citations

Abstract

Reactivation of human cytomegalovirus (HCMV) results in severe disease in AIDS patients and immunocompromised patients receiving blood transfusions or organ or bone marrow grafts. Although the site of HCMV latency is unknown, blood cells have been implicated as a viral reservoir. In this study, we demonstrate HCMV reactivation in vitro from seven consecutive healthy donors through allogeneic stimulation of peripheral blood mononuclear cells (PBMCs). HCMV replication was detected at 17 days poststimulation, and virus was recovered after long-term culture from a macrophage expressing dendritic cell markers. Thus, these observations demonstrate that PBMCs harbor latent HCMV, which reactivates in a myeloid lineage cell upon allogeneic stimulation.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalCell
Volume91
Issue number1
DOIs
StatePublished - Oct 3 1997

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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