Rates of incorporation of radioactive molecules during the cell cycle

Joe Gray, M. G. Pallavicini, Y. S. George, V. Groppi, M. Look, P. N. Dean

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The authors report measurements of the incorporation of radioactive molecules during short labeling periods, as a function of cell-cycle stage, using a cell-sorter-based technique that does not require cell synchronization. The authors determined: (1) tritiated thymidine ( 3H-TdR) incorporation throughout S-phase in Lewis lung tumor cells in vitro both before and after treatment with cytosine arabinoside; (2) 3H-TdR incorporation throughout S-phase in KHT tumor cells in vitro and in vivo; (3) 3H-TdR incorporation throughout S-phase in Chinese hamster ovary cells and compared it with DNA synthesis throughout S-phase; (4) a mathematical expression describing 3H-TdR incorporation throughout S-phase in Chinese hamster M3-1 cells; and (5) the simultaneous incorporation of 3H-TdR and 35S-methionine as they are related to cell size and DNA content in S49 mouse lymphoma cells. In asynchronously growing cells in vitro and in vivo, 3H-TdR incorporation was generally low in early and late S-phase and highest in mid-S-phase. However, in Lewis lung tumor cells treated with cytosine arabinoside 3H-TdR incorporation was highest in early and late S-phase and lowest in mid-S-phase. Incorporation of 35S-methionine increased continuously with cell size and DNA content. Incorporation of 3H-TdR in CHO cells was proportional to DNA synthesis.

Original languageEnglish (US)
Pages (from-to)135-144
Number of pages10
JournalJournal of Cellular Physiology
Volume108
Issue number2
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

S Phase
Cell Cycle
Cells
Molecules
Tumors
DNA
Cytarabine
Methionine
Cricetulus
Cell Size
Thymidine
Labeling
Synchronization
Neoplasms
Lung
CHO Cells
Ovary
Lymphoma

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Gray, J., Pallavicini, M. G., George, Y. S., Groppi, V., Look, M., & Dean, P. N. (1981). Rates of incorporation of radioactive molecules during the cell cycle. Journal of Cellular Physiology, 108(2), 135-144. https://doi.org/10.1002/jcp.1041080204

Rates of incorporation of radioactive molecules during the cell cycle. / Gray, Joe; Pallavicini, M. G.; George, Y. S.; Groppi, V.; Look, M.; Dean, P. N.

In: Journal of Cellular Physiology, Vol. 108, No. 2, 1981, p. 135-144.

Research output: Contribution to journalArticle

Gray, J, Pallavicini, MG, George, YS, Groppi, V, Look, M & Dean, PN 1981, 'Rates of incorporation of radioactive molecules during the cell cycle', Journal of Cellular Physiology, vol. 108, no. 2, pp. 135-144. https://doi.org/10.1002/jcp.1041080204
Gray, Joe ; Pallavicini, M. G. ; George, Y. S. ; Groppi, V. ; Look, M. ; Dean, P. N. / Rates of incorporation of radioactive molecules during the cell cycle. In: Journal of Cellular Physiology. 1981 ; Vol. 108, No. 2. pp. 135-144.
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