Rat mammary extracellular matrix composition and response to ibuprofen treatment during postpartum involution by differential GeLC-MS/MS analysis

Jenean H. OBrien, Lauren A. Vanderlinden, Pepper Schedin, Kirk C. Hansen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Breast cancer patients diagnosed within five years following pregnancy have increased metastasis and decreased survival. A hallmark of postpartum biology that may contribute to this poor prognosis is mammary gland involution, involving massive epithelial cell death and dramatic stromal remodeling. Previous studies show pro-tumorigenic properties of extracellular matrix (ECM) isolated from rodent mammary glands undergoing postpartum involution. More recent work demonstrates systemic ibuprofen treatment during involution decreases its tumor-promotional nature. Utilizing a proteomics approach, we identified relative differences in the composition of mammary ECM isolated from nulliparous rats and those undergoing postpartum involution, with and without ibuprofen treatment. GeLC-MS/MS experiments resulted in 20327 peptide identifications that mapped to 884 proteins with a

Original languageEnglish (US)
Pages (from-to)4894-4905
Number of pages12
JournalJournal of Proteome Research
Volume11
Issue number10
DOIs
StatePublished - Oct 5 2012
Externally publishedYes

Fingerprint

Ibuprofen
Postpartum Period
Extracellular Matrix
Rats
Breast
Human Mammary Glands
Cell death
Chemical analysis
Tumors
Proteomics
Peptides
Rodentia
Cell Death
Therapeutics
Epithelial Cells
Breast Neoplasms
Neoplasm Metastasis
Pregnancy
Proteins
Survival

Keywords

  • cancer biology
  • extracellular matrix
  • protein identification
  • quantification
  • stroma
  • tumor microenvironment

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Rat mammary extracellular matrix composition and response to ibuprofen treatment during postpartum involution by differential GeLC-MS/MS analysis. / OBrien, Jenean H.; Vanderlinden, Lauren A.; Schedin, Pepper; Hansen, Kirk C.

In: Journal of Proteome Research, Vol. 11, No. 10, 05.10.2012, p. 4894-4905.

Research output: Contribution to journalArticle

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