Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells

Carmen C. Baca Jones, Craig N. Kreklywich, Ilhem Messaoudi, Jennifer Vomaske, Erin McCartney, Susan Orloff, Jay Nelson, Daniel Streblow

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

While cytomegalovirus (CMV) infects and replicates in a multitude of cell types, the ability of the virus to replicate in antigen presenting cells (APCs) is believed to play a critical role in the viral dissemination and latency. CMV infection of APCs and manipulation of their function are important areas of investigation. CMV down regulation of MHC II is reportedly mediated by the HCMV proteins US2, US3, UL83, UL111a (vIL10) or through the induction of cellular IL10. In this study, we demonstrate that rat CMV (RCMV) significantly reduces MHC II expression neither by mechanisms that do not involve orthologues of the known HCMV genes nor by an increase in cellular IL10. Rat bone marrow derived dendritic cells (BMDC) were highly susceptible to infection with RCMV and a recombinant RCMV expressing eGFP. RCMV infection of BMDCs depleted both surface and intracellular MHC II to nearly undetectable levels as well as reduced surface expression of MHC I. The effect on MHC II only occurred in the infected GFP positive cells and is mediated by an immediate early or early viral gene product. Furthermore, treatment of uninfected immature DCs with virus-free conditioned supernatants from infected cells failed to down regulate MHC II. RCMV depletion of MHC II was sensitive to treatment with lysosomal inhibitors but not proteasomal inhibitors suggesting that the mechanism of RCMV-mediated down regulation of MHC II occurs through endocytic degradation. Since RCMV does not encode homologues of US2, US3, UL83 or UL111a, these data indicate a novel mechanism for RCMV depletion of MHC II.

Original languageEnglish (US)
Pages (from-to)78-90
Number of pages13
JournalVirology
Volume388
Issue number1
DOIs
StatePublished - May 25 2009

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Muromegalovirus
Cytomegalovirus Infections
Dendritic Cells
Bone Marrow
Down-Regulation
Antigen-Presenting Cells
Cytomegalovirus
Interleukin-10
Virus Latency
Viruses
Viral Proteins
Infection

Keywords

  • Antigen presentation/processing
  • Cytomegalovirus
  • Dendritic cells
  • MHC
  • Viral

ASJC Scopus subject areas

  • Virology

Cite this

Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. / Baca Jones, Carmen C.; Kreklywich, Craig N.; Messaoudi, Ilhem; Vomaske, Jennifer; McCartney, Erin; Orloff, Susan; Nelson, Jay; Streblow, Daniel.

In: Virology, Vol. 388, No. 1, 25.05.2009, p. 78-90.

Research output: Contribution to journalArticle

Baca Jones CC, Kreklywich CN, Messaoudi I, Vomaske J, McCartney E, Orloff S et al. Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. Virology. 2009 May 25;388(1):78-90. https://doi.org/10.1016/j.virol.2009.02.050
Baca Jones, Carmen C. ; Kreklywich, Craig N. ; Messaoudi, Ilhem ; Vomaske, Jennifer ; McCartney, Erin ; Orloff, Susan ; Nelson, Jay ; Streblow, Daniel. / Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. In: Virology. 2009 ; Vol. 388, No. 1. pp. 78-90.
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