RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation

Philippe P. Roux, David Shahbazian, Hieu Vu, Marina K. Holz, Michael Cohen, Jack Taunton, Nahum Sonenberg, John Blenis

Research output: Contribution to journalArticle

426 Citations (Scopus)

Abstract

Converging signals from the mammalian target of rapamycin (mTOR) and phosphoinositide 3-kinase (PI3K) pathways are well established to modulate translation initiation. Less is known regarding the molecular basis of protein synthesis regulated by other inputs, such as agonists of the Ras/extracellular signal-regulated kinase (ERK) signaling cascade. Ribosomal protein (rp) S6 is a component of the 40S ribosomal subunit that becomes phosphorylated at several serine residues upon mitogen stimulation, but the exact molecular mechanisms regulating its phosphorylation and the function of phosphorylated rpS6 is poorly understood. Here, we provide evidence that activation of the p90 ribosomal S6 kinases (RSKs) by serum, growth factors, tumor promoting phorbol esters, and oncogenic Ras is required for rpS6 phosphorylation downstream of the Ras/ERK signaling cascade. We demonstrate that while ribosomal S6 kinase 1 (S6K1) phosphorylates rpS6 at all sites, RSK exclusively phosphorylates rpS6 at Ser235/236 in vitro and in vivo using an mTOR-independent mechanism. Mutation of rpS6 at Ser235/236 reveals that phosphorylation of these sites promotes its recruitment to the 7-methylguanosine cap complex, suggesting that Ras/ERK signaling regulates assembly of the translation preinitiation complex. These data demonstrate that RSK provides an mTOR-independent pathway linking the Ras/ERK signaling cascade to the translational machinery.

Original languageEnglish (US)
Pages (from-to)14056-14064
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number19
DOIs
StatePublished - May 11 2007
Externally publishedYes

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Ribosomal Protein S6
Ribosomal Protein S6 Kinases
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Sirolimus
90-kDa Ribosomal Protein S6 Kinases
Eukaryotic Small Ribosome Subunits
1-Phosphatidylinositol 4-Kinase
Phorbol Esters
Phosphatidylinositols
Mitogens
Serine
Machinery
Tumors
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Chemical activation
Mutation
Serum
Neoplasms

ASJC Scopus subject areas

  • Biochemistry

Cite this

RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation. / Roux, Philippe P.; Shahbazian, David; Vu, Hieu; Holz, Marina K.; Cohen, Michael; Taunton, Jack; Sonenberg, Nahum; Blenis, John.

In: Journal of Biological Chemistry, Vol. 282, No. 19, 11.05.2007, p. 14056-14064.

Research output: Contribution to journalArticle

Roux, Philippe P. ; Shahbazian, David ; Vu, Hieu ; Holz, Marina K. ; Cohen, Michael ; Taunton, Jack ; Sonenberg, Nahum ; Blenis, John. / RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 19. pp. 14056-14064.
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