Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein

Sunwen Chou, K. M. Scott

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

An overnight assay, based on staining cytomegalovirus-infected cells with monoclonal antibody to the 72,000-molecular-weight major immediate-early viral protein, was compared with a conventional 14-day plaque assay for quantitation of cell-free stocks of cytomegalovirus laboratory strain AD-169 and 20 other clinical strains. Viral titers were quantitatively similar when determined by either method, but centrifugation of monolayers during inoculation enhanced viral infectivity an average of 4.1-fold. When used for scoring neutralizing antibody assays, monoclonal staining yielded titers within one dilution of 14-day plaque-reduction assays in 54 of 56 titrations. Of 21 cytomegalovirus strains, 2 were not recognized by the monoclonal antibody used. Assay with monoclonal antibody offers a rapid and accurate alternative to plaque assay for quantitation or neutralization of cytomegalovirus.

Original languageEnglish (US)
Pages (from-to)504-507
Number of pages4
JournalJournal of Clinical Microbiology
Volume26
Issue number3
StatePublished - 1988

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Immediate-Early Proteins
Viral Proteins
Neutralizing Antibodies
Cytomegalovirus
Monoclonal Antibodies
Staining and Labeling
Centrifugation
Molecular Weight

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

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