Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns

Tom Darling, Joseph Albert, Paul Russell, Daniel Albert, Ted W. Reid

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

High-performance steric exclusion chromatography on a 1250-Å pore size polyethylene glycol-treated glass bead column was used to purify avian myeloblastosis virus and hamster melanoma virus from plasma protein and tissue culture media. The purified hamster melanoma virus was still infectious and the avian myeloblastosis virus-associated RNA-directed DNA polymerase showed a 1100-fold purification of the virus from one column treatment. Electron microscopy of the purified virus showed intact particles, with surface projections evident. The time required for column purification of the virus was 5 min.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalJournal of Chromatography A
Volume131
Issue numberC
DOIs
StatePublished - Jan 21 1977
Externally publishedYes

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Chromatography
Viruses
Purification
Tumors
RNA
Glass
Proteins
Distillation columns
Tissue culture
RNA-Directed DNA Polymerase
Electron microscopy
Pore size
Culture Media
Blood Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns. / Darling, Tom; Albert, Joseph; Russell, Paul; Albert, Daniel; Reid, Ted W.

In: Journal of Chromatography A, Vol. 131, No. C, 21.01.1977, p. 383-390.

Research output: Contribution to journalArticle

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