Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns

Tom Darling; Joseph Albert; Paul Russell; Daniel M. Albert; Ted W. Reid

doi:10.1016/S0021-9673(00)80955-3

Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns

Tom Darling, Joseph Albert, Paul Russell, Daniel M. Albert, Ted W. Reid

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

High-performance steric exclusion chromatography on a 1250-Å pore size polyethylene glycol-treated glass bead column was used to purify avian myeloblastosis virus and hamster melanoma virus from plasma protein and tissue culture media. The purified hamster melanoma virus was still infectious and the avian myeloblastosis virus-associated RNA-directed DNA polymerase showed a 1100-fold purification of the virus from one column treatment. Electron microscopy of the purified virus showed intact particles, with surface projections evident. The time required for column purification of the virus was 5 min.

Original language	English (US)
Pages (from-to)	383-390
Number of pages	8
Journal	Journal of Chromatography A
Volume	131
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(00)80955-3
State	Published - Jan 21 1977
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(00)80955-3

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title = "Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns",

abstract = "High-performance steric exclusion chromatography on a 1250-{\AA} pore size polyethylene glycol-treated glass bead column was used to purify avian myeloblastosis virus and hamster melanoma virus from plasma protein and tissue culture media. The purified hamster melanoma virus was still infectious and the avian myeloblastosis virus-associated RNA-directed DNA polymerase showed a 1100-fold purification of the virus from one column treatment. Electron microscopy of the purified virus showed intact particles, with surface projections evident. The time required for column purification of the virus was 5 min.",

author = "Tom Darling and Joseph Albert and Paul Russell and Albert, {Daniel M.} and Reid, {Ted W.}",

note = "Funding Information: We thank Dr. Joseph Beard for the avian myeloblastosis virus and Dr. Gerald Hawk for helpful discussions. Supported by PHS grant from the National Eye Institute, NIH training grant for T.D. and RPB Research Professorship for T.W.R.",

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T1 - Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns

AU - Darling, Tom

AU - Albert, Joseph

AU - Russell, Paul

AU - Albert, Daniel M.

AU - Reid, Ted W.

N1 - Funding Information: We thank Dr. Joseph Beard for the avian myeloblastosis virus and Dr. Gerald Hawk for helpful discussions. Supported by PHS grant from the National Eye Institute, NIH training grant for T.D. and RPB Research Professorship for T.W.R.

PY - 1977/1/21

Y1 - 1977/1/21

N2 - High-performance steric exclusion chromatography on a 1250-Å pore size polyethylene glycol-treated glass bead column was used to purify avian myeloblastosis virus and hamster melanoma virus from plasma protein and tissue culture media. The purified hamster melanoma virus was still infectious and the avian myeloblastosis virus-associated RNA-directed DNA polymerase showed a 1100-fold purification of the virus from one column treatment. Electron microscopy of the purified virus showed intact particles, with surface projections evident. The time required for column purification of the virus was 5 min.

AB - High-performance steric exclusion chromatography on a 1250-Å pore size polyethylene glycol-treated glass bead column was used to purify avian myeloblastosis virus and hamster melanoma virus from plasma protein and tissue culture media. The purified hamster melanoma virus was still infectious and the avian myeloblastosis virus-associated RNA-directed DNA polymerase showed a 1100-fold purification of the virus from one column treatment. Electron microscopy of the purified virus showed intact particles, with surface projections evident. The time required for column purification of the virus was 5 min.

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Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns

Abstract

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