Rapid Point-of-Care Test for Determination of C-Peptide Levels

Paturi V. Rao, Eric Bean, Dhanalakshmi Nair-Schaef, Siting Chen, Steven C. Kazmierczak, Charles T. Roberts, Srinivasa R. Nagalla

Research output: Contribution to journalArticlepeer-review

Abstract

C-peptide is co-secreted with insulin and is not subject to hepatic clearance and thus reflects functional β-cell mass. Assessment of C-peptide levels can identify individuals at risk for or with type 1 diabetes with residual β-cell function in whom β cell-sparing interventions can be evaluated, and can aid in distinguishing type 2 diabetes from Latent Autoimmune Diabetes in Adults and late-onset type 1 diabetes. To facilitate C-peptide testing, we describe a quantitative point-of-care C-peptide test. C-peptide levels as low as 0.2 ng/ml were measurable in a fingerstick sample, and the test was accurate over a range of 0.17 to 12.0 ng/ml. This test exhibited a correlation of r = 0.98 with a high-sensitivity commercial ELISA assay and a correlation of r = 0.90 between matched serum and fingerstick samples.

Original languageEnglish (US)
JournalJournal of Diabetes Science and Technology
DOIs
StateAccepted/In press - 2021
Externally publishedYes

Keywords

  • C-peptide
  • LADA
  • MODY
  • diabetes
  • point-of-care

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Bioengineering
  • Biomedical Engineering

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