Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck

Hyo Sang Lee, Hong Ma, Rita Cervera Juanes, Masahito Tachibana, Michelle Sparman, Joy Woodward, Cathy Ramsey, Jing Xu, Eun Ju Kang, Paula Amato, Georg Mair, Ralf Steinborn, Shoukhrat Mitalipov

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The timing and mechanisms of mitochondrial DNA (mtDNA) segregation and transmission in mammals are poorly understood. Genetic bottleneck in female germ cells has been proposed as the main phenomenon responsible for rapid intergenerational segregation of heteroplasmic mtDNA. We demonstrate here that mtDNA segregation occurs during primate preimplantation embryogenesis resulting in partitioning of mtDNA variants between daughter blastomeres. A substantial shift toward homoplasmy occurred in fetuses and embryonic stem cells (ESCs) derived from these heteroplasmic embryos. We also observed a wide range of heteroplasmic mtDNA variants distributed in individual oocytes recovered from these fetuses. Thus, we present here evidence for a previously unknown mtDNA segregation and bottleneck during preimplantation embryo development, suggesting that return to the homoplasmic condition can occur during development of an individual organism from the zygote to birth, without a passage through the germline.

Original languageEnglish (US)
Pages (from-to)506-515
Number of pages10
JournalCell Reports
Volume1
Issue number5
DOIs
StatePublished - May 31 2012

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Blastocyst
Mitochondrial DNA
Primates
Embryonic Development
Fetus
Blastomeres
Mammals
Zygote
Embryonic Stem Cells
Stem cells
Germ Cells
Oocytes
Embryonic Structures
Cells
Parturition

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck. / Lee, Hyo Sang; Ma, Hong; Juanes, Rita Cervera; Tachibana, Masahito; Sparman, Michelle; Woodward, Joy; Ramsey, Cathy; Xu, Jing; Kang, Eun Ju; Amato, Paula; Mair, Georg; Steinborn, Ralf; Mitalipov, Shoukhrat.

In: Cell Reports, Vol. 1, No. 5, 31.05.2012, p. 506-515.

Research output: Contribution to journalArticle

Lee, HS, Ma, H, Juanes, RC, Tachibana, M, Sparman, M, Woodward, J, Ramsey, C, Xu, J, Kang, EJ, Amato, P, Mair, G, Steinborn, R & Mitalipov, S 2012, 'Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck', Cell Reports, vol. 1, no. 5, pp. 506-515. https://doi.org/10.1016/j.celrep.2012.03.011
Lee, Hyo Sang ; Ma, Hong ; Juanes, Rita Cervera ; Tachibana, Masahito ; Sparman, Michelle ; Woodward, Joy ; Ramsey, Cathy ; Xu, Jing ; Kang, Eun Ju ; Amato, Paula ; Mair, Georg ; Steinborn, Ralf ; Mitalipov, Shoukhrat. / Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck. In: Cell Reports. 2012 ; Vol. 1, No. 5. pp. 506-515.
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