Rapid metabolic clearance of the catechol estrogen 2-hydroxyestrone

George R. Merriam; David D. Brandon; Shinzo Kono; S. Edward Davis; D. Lynn Loriaux; Mdrtimer B. Lipsett

doi:10.1210/jcem-51-5-1211

Rapid metabolic clearance of the catechol estrogen 2-hydroxyestrone

George R. Merriam, David D. Brandon, Shinzo Kono, S. Edward Davis, D. Lynn Loriaux, Mdrtimer B. Lipsett

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The plasma metabolic clearance rate (MCR_p) of 2-hydroxyestrone was measured in normal young adults by two methods: infusion of unlabeled 2-OHE₁ to equilibrium with radioimmunoassay of plasma 2-OHE₁ levels, and infusion of [³H]2-OHE₁ to equilibrium with measurement of chromatographically purified plasma [³H]2-OHE₁. MCR's were 40-70,000 L/day and 15-50,000 L/day, respectively. This is the highest known MCR for a naturally occurring steroid. The only measurable plasma metabolite of [³H]2-OHE₁ co-migrated with 2-methoxyestrone. When [³H]2-OHE₁ was incubated with blood samples in vitro, [³H]ξ-methoxyestrone was rapidly formed. Since this MCR is higher than cardiac output, clearance must occur primarily in the blood compartment, probably largely by the action of erythrocyte catechol-0-methyltransferase.

Original language	English (US)
Pages (from-to)	1211-1213
Number of pages	3
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	51
Issue number	5
DOIs	https://doi.org/10.1210/jcem-51-5-1211
State	Published - Nov 1980
Externally published	Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Endocrinology
Clinical Biochemistry
Biochemistry, medical

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title = "Rapid metabolic clearance of the catechol estrogen 2-hydroxyestrone",

abstract = "The plasma metabolic clearance rate (MCRp) of 2-hydroxyestrone was measured in normal young adults by two methods: infusion of unlabeled 2-OHE1 to equilibrium with radioimmunoassay of plasma 2-OHE1 levels, and infusion of [3H]2-OHE1 to equilibrium with measurement of chromatographically purified plasma [3H]2-OHE1. MCR's were 40-70,000 L/day and 15-50,000 L/day, respectively. This is the highest known MCR for a naturally occurring steroid. The only measurable plasma metabolite of [3H]2-OHE1 co-migrated with 2-methoxyestrone. When [3H]2-OHE1 was incubated with blood samples in vitro, [3H]ξ-methoxyestrone was rapidly formed. Since this MCR is higher than cardiac output, clearance must occur primarily in the blood compartment, probably largely by the action of erythrocyte catechol-0-methyltransferase.",

author = "Merriam, {George R.} and Brandon, {David D.} and Shinzo Kono and Davis, {S. Edward} and Loriaux, {D. Lynn} and Lipsett, {Mdrtimer B.}",

year = "1980",

month = nov,

doi = "10.1210/jcem-51-5-1211",

language = "English (US)",

volume = "51",

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journal = "Journal of Clinical Endocrinology and Metabolism",

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T1 - Rapid metabolic clearance of the catechol estrogen 2-hydroxyestrone

AU - Merriam, George R.

AU - Brandon, David D.

AU - Kono, Shinzo

AU - Davis, S. Edward

AU - Loriaux, D. Lynn

AU - Lipsett, Mdrtimer B.

PY - 1980/11

Y1 - 1980/11

N2 - The plasma metabolic clearance rate (MCRp) of 2-hydroxyestrone was measured in normal young adults by two methods: infusion of unlabeled 2-OHE1 to equilibrium with radioimmunoassay of plasma 2-OHE1 levels, and infusion of [3H]2-OHE1 to equilibrium with measurement of chromatographically purified plasma [3H]2-OHE1. MCR's were 40-70,000 L/day and 15-50,000 L/day, respectively. This is the highest known MCR for a naturally occurring steroid. The only measurable plasma metabolite of [3H]2-OHE1 co-migrated with 2-methoxyestrone. When [3H]2-OHE1 was incubated with blood samples in vitro, [3H]ξ-methoxyestrone was rapidly formed. Since this MCR is higher than cardiac output, clearance must occur primarily in the blood compartment, probably largely by the action of erythrocyte catechol-0-methyltransferase.

AB - The plasma metabolic clearance rate (MCRp) of 2-hydroxyestrone was measured in normal young adults by two methods: infusion of unlabeled 2-OHE1 to equilibrium with radioimmunoassay of plasma 2-OHE1 levels, and infusion of [3H]2-OHE1 to equilibrium with measurement of chromatographically purified plasma [3H]2-OHE1. MCR's were 40-70,000 L/day and 15-50,000 L/day, respectively. This is the highest known MCR for a naturally occurring steroid. The only measurable plasma metabolite of [3H]2-OHE1 co-migrated with 2-methoxyestrone. When [3H]2-OHE1 was incubated with blood samples in vitro, [3H]ξ-methoxyestrone was rapidly formed. Since this MCR is higher than cardiac output, clearance must occur primarily in the blood compartment, probably largely by the action of erythrocyte catechol-0-methyltransferase.

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Rapid metabolic clearance of the catechol estrogen 2-hydroxyestrone

Abstract

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