@article{f90ba8a643bf4e96b27464fe1a841205,
title = "Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition",
abstract = "We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.",
author = "Andrew Adey and Morrison, {Hilary G.} and Asan and Xu Xun and Kitzman, {Jacob O.} and Turner, {Emily H.} and Bethany Stackhouse and MacKenzie, {Alexandra P.} and Caruccio, {Nicholas C.} and Xiuqing Zhang and Jay Shendure",
note = "Funding Information: We would like to acknowledge W Reznikoff for conceptual contributions; H Grunenwald and others at Epicentre for early access to materials; C Lee, J Hiatt, R Patwardhan, L Felker, R Qiu, B O{\textquoteright}Roak, and other members of the Shendure Lab for assistance with protocol development and sequencing; the genome sequencing platform of BGI-Shenzhen, especially Wu Kui, Jiang Hui, Chen Mingfeng, Liu Lin, Yu Lili and Kaileung Yuan, for their contributions to YH1 and Drosophila genome sequencing; He Weiming, Liu Xingwang and Yang Lei for their genotyping work; and I. Meek and E. Wong-Ho (Caliper) for size selections on the LabChip XT. This work was supported in part by grants from the National Institutes of Health/National Heart Lung and Blood Institute (R01 HL094976 to JS), the National Institutes of Health/National Human Genome Research Institute (R21 HG004749 to JS), the National Institutes of Health/National Institute of Allergy and Infectious Disease Northwest Regional Center of Excellence for Biodefense and Emerging Infectious Diseases at the University of Washington (3U54AI05714), the Ministry of Science and Technology of China, 863 program (2006AA02A301), and an NSF Graduate Research Fellowship (to JOK).",
year = "2010",
month = dec,
day = "8",
doi = "10.1186/gb-2010-11-12-r119",
language = "English (US)",
volume = "11",
journal = "Genome Biology",
issn = "1474-7596",
publisher = "BioMed Central",
number = "12",
}