Rapid inertial solution exchange for enrichment and flow cytometric detection of microvesicles

Jaideep S. Dudani, Daniel R. Gossett, Henry T.K. Tse, Robert J. Lamm, Rajan P. Kulkarni, Dino Di Carlo

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Exosomes, nanosized membrane-bound vesicles released by cells, play roles in cell signaling, immunology, virology, and oncology. Their study, however, has been hampered by difficulty in isolation and quantification due to their size and the complexity of biological samples. Conventional approaches to improved isolation require specialized equipment and extensive sample preparation time. Therefore, isolation and detection methods of exosomes will benefit biological and clinical studies. Here, we report a microfluidic platform for inline exosome isolation and fluorescent detection using inertial manipulation of antibody-coated exosome capture beads from biological fluids.

Original languageEnglish (US)
Article number014112
JournalBiomicrofluidics
Volume9
Issue number1
DOIs
StatePublished - Feb 5 2015
Externally publishedYes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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