Rapid human chromosome aberration analysis using fluorescence in situ hybridization

J. N. Lucas, T. Tenjin, T. Straume, D. Pinkel, D. Moore, M. Litt, Joe Gray

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

We have used in situ hybridization of repeat-sequence DNA probes, specific to the paracentromeric locus 1q12 and the telomeric locus 1p36, to fluorescently stain regions that flank human chromosome 1p. This procedure was used for fast detection of structural aberrations involving human chromosome 1p in two separate experiments. In one, human lymphocytes were irradiated with 0, 0·8, 1·6, 2·4 and 3·2 Gy of 137Cs γrays. In the other, human lymphocytes were irradiated with 0, 0·09, 0·18, 2·0, 3·1 and 4·1 Gy of 60Co γrays. The frequencies (per cell) of translocations and dicentrics with one breakpoint in 1p and one elsewhere in the genome were determined for cells irradiated at each dose point. These frequencies both increased with dose, D, in a linear-quadratic manner. The δ α and β coefficients resulting from a fit of the equation f(D) = δ + αD + βD2 to the translocation frequency dose-response data were 0·0025, 0·0027 and 0·0037 for 137Cs γrays, and 0·0010, 0·0041, and 0·0057 for 60Co γrays. The δ α and β coefficients resulting from a fit to the dicentric frequency dose-response data were 0·0005, 0·0010 and 0·0028 for 137Cs γrays and 0·0001, 0·0002 and 0·0035, for 60Co γrays. Approximately 32,000 metaphase spreads were scored in this study. The average analysis rate was over two metaphase spreads per minute. However, an experienced analyst was able to find and score one metaphase spread every 10 s. The importance of this new cytogenetic analysis technique for biological dosimetry and in vivo risk assessment is discussed.

Original languageEnglish (US)
Pages (from-to)35-44
Number of pages10
JournalInternational Journal of Radiation Biology
Volume56
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

chromosome aberrations
Lymphocytes
Human Chromosomes
Metaphase
Chromosomes
fluorescence in situ hybridization
Aberrations
Fluorescence In Situ Hybridization
Chromosome Aberrations
rays
Cytogenetic Analysis
Fluorescence
metaphase
fluorescence
DNA sequences
Risk assessment
Dosimetry
dose response
dosage
lymphocytes

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Rapid human chromosome aberration analysis using fluorescence in situ hybridization. / Lucas, J. N.; Tenjin, T.; Straume, T.; Pinkel, D.; Moore, D.; Litt, M.; Gray, Joe.

In: International Journal of Radiation Biology, Vol. 56, No. 1, 1989, p. 35-44.

Research output: Contribution to journalArticle

Lucas, J. N. ; Tenjin, T. ; Straume, T. ; Pinkel, D. ; Moore, D. ; Litt, M. ; Gray, Joe. / Rapid human chromosome aberration analysis using fluorescence in situ hybridization. In: International Journal of Radiation Biology. 1989 ; Vol. 56, No. 1. pp. 35-44.
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