TY - JOUR
T1 - Rapid human chromosome aberration analysis using fluorescence in situ hybridization
AU - Lucas, J. N.
AU - Tenjin, T.
AU - Straume, T.
AU - Pinkel, D.
AU - Moore, D.
AU - Litt, M.
AU - Gray, J. W.
N1 - Funding Information:
Acknowledgements The authors thank Dr James Tucker and Ms Mari Christensen for scoring translocation frequencies using G-banding . Work performed under the auspices of the U .S . Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48 with support from USPHS grant GM25076 .
PY - 1989
Y1 - 1989
N2 - We have used in situ hybridization of repeat-sequence DNA probes, specific to the paracentromeric locus 1q12 and the telomeric locus 1p36, to fluorescently stain regions that flank human chromosome 1p. This procedure was used for fast detection of structural aberrations involving human chromosome 1p in two separate experiments. In one, human lymphocytes were irradiated with 0, 0·8, 1·6, 2·4 and 3·2 Gy of 137Cs γrays. In the other, human lymphocytes were irradiated with 0, 0·09, 0·18, 2·0, 3·1 and 4·1 Gy of 60Co γrays. The frequencies (per cell) of translocations and dicentrics with one breakpoint in 1p and one elsewhere in the genome were determined for cells irradiated at each dose point. These frequencies both increased with dose, D, in a linear-quadratic manner. The δ α and β coefficients resulting from a fit of the equation f(D) = δ + αD + βD2 to the translocation frequency dose-response data were 0·0025, 0·0027 and 0·0037 for 137Cs γrays, and 0·0010, 0·0041, and 0·0057 for 60Co γrays. The δ α and β coefficients resulting from a fit to the dicentric frequency dose-response data were 0·0005, 0·0010 and 0·0028 for 137Cs γrays and 0·0001, 0·0002 and 0·0035, for 60Co γrays. Approximately 32,000 metaphase spreads were scored in this study. The average analysis rate was over two metaphase spreads per minute. However, an experienced analyst was able to find and score one metaphase spread every 10 s. The importance of this new cytogenetic analysis technique for biological dosimetry and in vivo risk assessment is discussed.
AB - We have used in situ hybridization of repeat-sequence DNA probes, specific to the paracentromeric locus 1q12 and the telomeric locus 1p36, to fluorescently stain regions that flank human chromosome 1p. This procedure was used for fast detection of structural aberrations involving human chromosome 1p in two separate experiments. In one, human lymphocytes were irradiated with 0, 0·8, 1·6, 2·4 and 3·2 Gy of 137Cs γrays. In the other, human lymphocytes were irradiated with 0, 0·09, 0·18, 2·0, 3·1 and 4·1 Gy of 60Co γrays. The frequencies (per cell) of translocations and dicentrics with one breakpoint in 1p and one elsewhere in the genome were determined for cells irradiated at each dose point. These frequencies both increased with dose, D, in a linear-quadratic manner. The δ α and β coefficients resulting from a fit of the equation f(D) = δ + αD + βD2 to the translocation frequency dose-response data were 0·0025, 0·0027 and 0·0037 for 137Cs γrays, and 0·0010, 0·0041, and 0·0057 for 60Co γrays. The δ α and β coefficients resulting from a fit to the dicentric frequency dose-response data were 0·0005, 0·0010 and 0·0028 for 137Cs γrays and 0·0001, 0·0002 and 0·0035, for 60Co γrays. Approximately 32,000 metaphase spreads were scored in this study. The average analysis rate was over two metaphase spreads per minute. However, an experienced analyst was able to find and score one metaphase spread every 10 s. The importance of this new cytogenetic analysis technique for biological dosimetry and in vivo risk assessment is discussed.
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U2 - 10.1080/09553008914551161
DO - 10.1080/09553008914551161
M3 - Article
C2 - 2569008
AN - SCOPUS:0024409797
SN - 0955-3002
VL - 56
SP - 35
EP - 44
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 1
ER -