Rapid determination of single base mismatch mutations in DNA hybrids by direct electric field control

Ronald G. Sosnowski, Eugene Tu, William F. Butler, James P. O'Connell, Michael (Mike) Heller

Research output: Contribution to journalArticle

400 Citations (Scopus)

Abstract

We have demonstrated that controlled electric fields can be used to regulate transport, concentration, hybridization, and denaturation of single- and double-stranded oligonucleotides. Discrimination among oligonucleotide hybrids with widely varying binding strengths may be attained by simple adjustment of the electric field strength. When this approach is used, electric field denaturation control allows single base pair mismatch discrimination to be carried out rapidly (<15 sec) and with high resolution. Electric field denaturation takes place at temperatures well below the melting point of the hybrids, and it may constitute a novel mechanism of DNA denaturation.

Original languageEnglish (US)
Pages (from-to)1119-1123
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number4
DOIs
StatePublished - Feb 18 1997
Externally publishedYes

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Oligonucleotides
Base Pair Mismatch
Nucleic Acid Denaturation
Mutation
DNA
Freezing
Temperature

ASJC Scopus subject areas

  • General

Cite this

Rapid determination of single base mismatch mutations in DNA hybrids by direct electric field control. / Sosnowski, Ronald G.; Tu, Eugene; Butler, William F.; O'Connell, James P.; Heller, Michael (Mike).

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 4, 18.02.1997, p. 1119-1123.

Research output: Contribution to journalArticle

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