Rapid detection of Salmonella enterica with primers specific for iroB

Andreas J. Bäumler, Fred Heffron, Rolf Reissbrodt

    Research output: Contribution to journalArticle

    82 Citations (Scopus)

    Abstract

    The iroB gene of Salmonella enterica is absent from the chromosome of the related organism Escherichia coli. We determined the distribution of this gene among 150 bacterial isolates, representing 51 serotypes of different Salmonella species and subspecies and 8 other bacterial species which are frequent contaminants during routine enrichment procedures by Southern hybridization. An iroB-specific DNA probe detected homologous sequences in all strains of S. enterica, including serotypes of S. enterica subsp. enterica (I), salamae (II), diarizonae (IIIb), and houtenae (IV). No hybridization signal was obtained with strains of Salmonella bongori or other bacterial species. In contrast, hybridization with a DNA probe specific for purD, a purine biosynthesis gene, detected homologs in all bacterial species tested. Primers specific for iroB were used to amplify this gene from 197 bacterial isolates by PCR. The iroB gene could be PCR amplified from S. enterica subsp. enterica (I), salamae (II), diarizonae (IIIb), houtenae (IV), arizonae (IIIa), and indica (VI), but not from S. bongori or other bacterial species. Thus, PCR amplification of iroB can be used to distinguish between S. enterica and other bacterial species, including S. bongori. A combination of preenrichment in buffered peptone water supplemented with ferrioxamine E and amplification of iroB by magnetic immuno-PCR allowed detection of S. enterica in albumen within 24 h. In conclusion, PCR amplification of iroB is a new sensitive and selective method which has the potential to rapidly detect S. enterica serotypes.

    Original languageEnglish (US)
    Pages (from-to)1224-1230
    Number of pages7
    JournalJournal of Clinical Microbiology
    Volume35
    Issue number5
    StatePublished - May 1997

    Fingerprint

    Salmonella enterica
    Polymerase Chain Reaction
    DNA Probes
    Salmonella
    Genes
    Bacterial Genes
    Peptones
    Sequence Homology
    Chromosomes
    Escherichia coli
    Water
    Serogroup

    ASJC Scopus subject areas

    • Microbiology (medical)
    • Microbiology

    Cite this

    Bäumler, A. J., Heffron, F., & Reissbrodt, R. (1997). Rapid detection of Salmonella enterica with primers specific for iroB. Journal of Clinical Microbiology, 35(5), 1224-1230.

    Rapid detection of Salmonella enterica with primers specific for iroB. / Bäumler, Andreas J.; Heffron, Fred; Reissbrodt, Rolf.

    In: Journal of Clinical Microbiology, Vol. 35, No. 5, 05.1997, p. 1224-1230.

    Research output: Contribution to journalArticle

    Bäumler, AJ, Heffron, F & Reissbrodt, R 1997, 'Rapid detection of Salmonella enterica with primers specific for iroB', Journal of Clinical Microbiology, vol. 35, no. 5, pp. 1224-1230.
    Bäumler, Andreas J. ; Heffron, Fred ; Reissbrodt, Rolf. / Rapid detection of Salmonella enterica with primers specific for iroB. In: Journal of Clinical Microbiology. 1997 ; Vol. 35, No. 5. pp. 1224-1230.
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    abstract = "The iroB gene of Salmonella enterica is absent from the chromosome of the related organism Escherichia coli. We determined the distribution of this gene among 150 bacterial isolates, representing 51 serotypes of different Salmonella species and subspecies and 8 other bacterial species which are frequent contaminants during routine enrichment procedures by Southern hybridization. An iroB-specific DNA probe detected homologous sequences in all strains of S. enterica, including serotypes of S. enterica subsp. enterica (I), salamae (II), diarizonae (IIIb), and houtenae (IV). No hybridization signal was obtained with strains of Salmonella bongori or other bacterial species. In contrast, hybridization with a DNA probe specific for purD, a purine biosynthesis gene, detected homologs in all bacterial species tested. Primers specific for iroB were used to amplify this gene from 197 bacterial isolates by PCR. The iroB gene could be PCR amplified from S. enterica subsp. enterica (I), salamae (II), diarizonae (IIIb), houtenae (IV), arizonae (IIIa), and indica (VI), but not from S. bongori or other bacterial species. Thus, PCR amplification of iroB can be used to distinguish between S. enterica and other bacterial species, including S. bongori. A combination of preenrichment in buffered peptone water supplemented with ferrioxamine E and amplification of iroB by magnetic immuno-PCR allowed detection of S. enterica in albumen within 24 h. In conclusion, PCR amplification of iroB is a new sensitive and selective method which has the potential to rapidly detect S. enterica serotypes.",
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