Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood

Michael J. Heller, Raj Krishnan, Avery Sonnenberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

The ability to rapidly detect cell free circulating (cfc) DNA, cfc-RNA, exosomes and other nanoparticulate disease biomarkers as well as drug delivery nanoparticles directly in blood is a major challenge for nanomedicine. We now show that microarray and new high voltage dielectrophoretic (DEP) devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates and nanoparticles directly from whole blood and other high conductance samples (plasma, serum, urine, etc.). At DEP frequencies of 5kHz-10kHz both fluorescent-stained high molecular weight (hmw) DNA, cfc-DNA and fluorescent nanoparticles separate from the blood and become highly concentrated at specific DEP highfield regions over the microelectrodes, while blood cells move to the DEP low field-regions. The blood cells can then be removed by a simple fluidic wash while the DNA and nanoparticles remain highly concentrated. The hmw-DNA could be detected at a level of <260ng/ml and the nanoparticles at <9.5 × 109 particles/ml, detection levels that are well within the range for viable clinical diagnostics and drug nanoparticle monitoring. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia (CLL) and confirmed by PCR genotyping analysis.

Original languageEnglish (US)
Title of host publicationBiosensing III
DOIs
StatePublished - Oct 18 2010
EventBiosensing III - San Diego, CA, United States
Duration: Aug 1 2010Aug 3 2010

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7759
ISSN (Print)0277-786X

Conference

ConferenceBiosensing III
CountryUnited States
CitySan Diego, CA
Period8/1/108/3/10

Keywords

  • Biomarkers
  • Blood
  • Cancer diagnostics
  • Cfc-DNA
  • Dielectrophoresis (DEP)
  • Exosomes
  • Nanoparticles
  • RNA

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood'. Together they form a unique fingerprint.

  • Cite this

    Heller, M. J., Krishnan, R., & Sonnenberg, A. (2010). Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood. In Biosensing III [77590P] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7759). https://doi.org/10.1117/12.861579