Rapid communication: Thyrotropin receptor/thyroglobulin messenger ribonucleic acid in peripheral blood and fine-needle aspiration cytology: Diagnostic synergy for detecting thyroid cancer

Kristin Wagner, Rosemarie Arciaga, Allan Siperstein, Kresimira Milas, Ilka Warshawsky, S. Sethu K Reddy, Manjula K. Gupta

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

RT-PCR for thyroglobulin (Tg) and TSH receptor (TSHR) mRNA has been used to detect circulating thyroid cancer cells. Little is known, however, regarding the preoperative sensitivity of this test to detect cancer. Seventy-two patients with thyroid disease (36 with malignancy and 36 with benign disease) were evaluated preoperatively. TSHR and Tg mRNA transcripts were detected by RT-PCR assays, previously determined to be specific for cancer cells. There was 100% concordance between TSHR and Tg mRNA RT-PCR results. Of 36 cancer patients, 11 had recurrent disease, and all were positive by RT-PCR. Among 25 patients with no prior thyroid surgery, 18 tested positive preoperatively (sensitivity 72%). Seven of 36 patients with benign disease tested positive (specificity 80%). The overall preoperative diagnostic accuracy was 77%. Preoperative fine-needle aspiration (FNA) biopsy was performed on 46 of 61 patients with no prior thyroid surgery. FNA was diagnostic in 28 (61%) patients. Preoperative cytology was adequate but not diagnostic in 18 (39%) patients. RT-PCR correctly classified 14 of these 18 patients with indeterminate FNA, and the test detected three of four cancer patients as positive (75% sensitive) and 11 of 14 patients (78% specific) with benign disease as negative. The combined diagnostic performance characteristics for RT-PCR and FNA cytology were sensitivity = 95%, specificity = 83%, and diagnostic accuracy = 89%, with positive and negative predictive values of 84 and 95%, respectively. Our results suggest that the molecular detection of circulating thyroid cancer cells by RT-PCR for TSHR/Tg mRNA complements FNA cytology in the preoperative differentiation of benign from malignant thyroid disease and their combined use may save unnecessary surgeries.

Original languageEnglish (US)
Pages (from-to)1921-1924
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume90
Issue number4
DOIs
StatePublished - Apr 2005
Externally publishedYes

Fingerprint

Cytology
Thyrotropin Receptors
Thyroglobulin
Fine Needle Biopsy
Thyroid Neoplasms
Needles
Cell Biology
Blood
Communication
RNA
Surgery
Polymerase Chain Reaction
Messenger RNA
Cells
Thyroid Diseases
Neoplasms
Biopsy
Thyroid Gland
Assays
Unnecessary Procedures

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Rapid communication : Thyrotropin receptor/thyroglobulin messenger ribonucleic acid in peripheral blood and fine-needle aspiration cytology: Diagnostic synergy for detecting thyroid cancer. / Wagner, Kristin; Arciaga, Rosemarie; Siperstein, Allan; Milas, Kresimira; Warshawsky, Ilka; Reddy, S. Sethu K; Gupta, Manjula K.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 90, No. 4, 04.2005, p. 1921-1924.

Research output: Contribution to journalArticle

Wagner, Kristin ; Arciaga, Rosemarie ; Siperstein, Allan ; Milas, Kresimira ; Warshawsky, Ilka ; Reddy, S. Sethu K ; Gupta, Manjula K. / Rapid communication : Thyrotropin receptor/thyroglobulin messenger ribonucleic acid in peripheral blood and fine-needle aspiration cytology: Diagnostic synergy for detecting thyroid cancer. In: Journal of Clinical Endocrinology and Metabolism. 2005 ; Vol. 90, No. 4. pp. 1921-1924.
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abstract = "RT-PCR for thyroglobulin (Tg) and TSH receptor (TSHR) mRNA has been used to detect circulating thyroid cancer cells. Little is known, however, regarding the preoperative sensitivity of this test to detect cancer. Seventy-two patients with thyroid disease (36 with malignancy and 36 with benign disease) were evaluated preoperatively. TSHR and Tg mRNA transcripts were detected by RT-PCR assays, previously determined to be specific for cancer cells. There was 100{\%} concordance between TSHR and Tg mRNA RT-PCR results. Of 36 cancer patients, 11 had recurrent disease, and all were positive by RT-PCR. Among 25 patients with no prior thyroid surgery, 18 tested positive preoperatively (sensitivity 72{\%}). Seven of 36 patients with benign disease tested positive (specificity 80{\%}). The overall preoperative diagnostic accuracy was 77{\%}. Preoperative fine-needle aspiration (FNA) biopsy was performed on 46 of 61 patients with no prior thyroid surgery. FNA was diagnostic in 28 (61{\%}) patients. Preoperative cytology was adequate but not diagnostic in 18 (39{\%}) patients. RT-PCR correctly classified 14 of these 18 patients with indeterminate FNA, and the test detected three of four cancer patients as positive (75{\%} sensitive) and 11 of 14 patients (78{\%} specific) with benign disease as negative. The combined diagnostic performance characteristics for RT-PCR and FNA cytology were sensitivity = 95{\%}, specificity = 83{\%}, and diagnostic accuracy = 89{\%}, with positive and negative predictive values of 84 and 95{\%}, respectively. Our results suggest that the molecular detection of circulating thyroid cancer cells by RT-PCR for TSHR/Tg mRNA complements FNA cytology in the preoperative differentiation of benign from malignant thyroid disease and their combined use may save unnecessary surgeries.",
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