Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing

Michael Rutten, Bryan Laraway, Cynthia Gregory, Hua Xie, Christian Renken, Charles Keese, Kenton Gregory

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Regenerative medicine studies using autologous bone marrow mononuclear cells (BM-MNCs) have shown improved clinical outcomes that correlate to in vitro BM-MNC invasive capacity. The current Boyden-chamber assay for testing invasive capacity is labor-intensive, provides only a single time point, and takes 36 hours to collect data and results, which is not practical from a clinical cell delivery perspective. To develop a rapid, sensitive and reproducible invasion assay, we employed Electric Cell-substrate Impedance Sensing (ECIS) technology. Chemokine-directed BM-MNC cell invasion across a Matrigel-coated Transwell filter was measurable within minutes using the ECIS system we developed. This ECIS-Transwell chamber system provides a rapid and sensitive test of stem and progenitor cell invasive capacity for evaluation of stem cell functionality to provide timely clinical data for selection of patients likely to realize clinical benefit in regenerative medicine treatments. This device could also supply robust unambiguous, reproducible and cost effective data as a potency assay for cell product release and regulatory strategies.

Original languageEnglish (US)
Article number182
JournalStem Cell Research and Therapy
Volume6
Issue number1
DOIs
StatePublished - Dec 1 2015

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Colony-Forming Units Assay
Electric batteries
Stem cells
Electric Impedance
Assays
Bone
Substrates
Bone Marrow Cells
Regenerative Medicine
Stem Cells
Chemokines
Personnel
Testing
Patient Selection
Costs
Technology
Costs and Cost Analysis
Equipment and Supplies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Medicine
  • Cell Biology
  • Medicine (miscellaneous)

Cite this

Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing. / Rutten, Michael; Laraway, Bryan; Gregory, Cynthia; Xie, Hua; Renken, Christian; Keese, Charles; Gregory, Kenton.

In: Stem Cell Research and Therapy, Vol. 6, No. 1, 182, 01.12.2015.

Research output: Contribution to journalArticle

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