Rapid and sensitive detection of nano-fluidically trapped protein biomarkers

Nandhinee Radha Shanmugam, Anjan Panneer Selvam, Thomas W. Barrett, Steven (Steve) Kazmierczak, Shalini Prasad

Research output: Contribution to journalArticle

Abstract

In this work, we demonstrate the label-free and ultrasensitive detection of troponin-T, cardiac biomarker using nanoporous membrane integrated on a microelectrode sensor platform. The nanoporous membrane allows for spatial confinement of the protein molecules. Antigen interaction with thiol immobilized antibody perturbs the electrical double layer. Charge perturbations are recorded as impedance change at low frequency using the principle of electrochemical impedance spectroscopy (EIS). The measured impedance change is used to quantitatively determine the concentration of troponin-T in tested sample. We have shown that sensitivity of sensor for troponin-T to be 1pg/mL. The accuracy and reliability of this sensor was tested by comparing the experimental troponin-T concentration values with a commercially available electrochemiluminescence assay measured with Roche Elecsys analyzer. Using this technique we were successful in detecting protein biomarkers in whole blood, human serum, and ionic buffers. This technology provides a robust analytical platform for rapid and sensitive detection of protein biomarkers, thus establishing this technology as an ideal candidate for biomarker screening in clinical settings.

Original languageEnglish (US)
JournalDisaster Medicine and Public Health Preparedness
Volume1686
Issue number6
DOIs
StatePublished - Nov 21 2014

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Troponin T
Biomarkers
Electric Impedance
Proteins
Immobilized Antibodies
Technology
Dielectric Spectroscopy
Membranes
Microelectrodes
Sulfhydryl Compounds
Buffers
Antigens
Serum

Keywords

  • nanoscale
  • porosity
  • sensor

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Rapid and sensitive detection of nano-fluidically trapped protein biomarkers. / Shanmugam, Nandhinee Radha; Selvam, Anjan Panneer; Barrett, Thomas W.; Kazmierczak, Steven (Steve); Prasad, Shalini.

In: Disaster Medicine and Public Health Preparedness, Vol. 1686, No. 6, 21.11.2014.

Research output: Contribution to journalArticle

Shanmugam, Nandhinee Radha ; Selvam, Anjan Panneer ; Barrett, Thomas W. ; Kazmierczak, Steven (Steve) ; Prasad, Shalini. / Rapid and sensitive detection of nano-fluidically trapped protein biomarkers. In: Disaster Medicine and Public Health Preparedness. 2014 ; Vol. 1686, No. 6.
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