Rapid and accurate large-scale genotyping of duplicated genes and discovery of interlocus gene conversions

Xander Nuttle, John Huddleston, Brian J. O'roak, Francesca Antonacci, Marco Fichera, Corrado Romano, Jay Shendure, Evan E. Eichler

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Over 900 genes have been annotated within duplicated regions of the human genome, yet their functions and potential roles in disease remain largely unknown. One major obstacle has been the inability to accurately and comprehensively assay genetic variation for these genes in a high-throughput manner. We developed a sequencing-based method for rapid and high-throughput genotyping of duplicated genes using molecular inversion probes designed to target unique paralogous sequence variants. We applied this method to genotype all members of two gene families, SRGAP2 and RH, among a diversity panel of 1,056 humans. The approach could accurately distinguish copy number in paralogs having up to ∼99.6% sequence identity, identify small gene-disruptive deletions, detect single-nucleotide variants, define breakpoints of unequal crossover and discover regions of interlocus gene conversion. The ability to rapidly and accurately genotype multiple gene families in thousands of individuals at low cost enables the development of genome-wide gene conversion maps and 'unlocks' many previously inaccessible duplicated genes for association with human traits.

Original languageEnglish (US)
Pages (from-to)903-909
Number of pages7
JournalNature Methods
Volume10
Issue number9
DOIs
StatePublished - Sep 1 2013

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Nuttle, X., Huddleston, J., O'roak, B. J., Antonacci, F., Fichera, M., Romano, C., Shendure, J., & Eichler, E. E. (2013). Rapid and accurate large-scale genotyping of duplicated genes and discovery of interlocus gene conversions. Nature Methods, 10(9), 903-909. https://doi.org/10.1038/nmeth.2572