Rapid actin-based plasticity in dendritic spines

Maria Fischer, Stefanie Kaech-Petrie, Darko Knutti, Andrew Matus

Research output: Contribution to journalArticle

734 Citations (Scopus)

Abstract

Dendritic spines have been proposed as primary sites of synaptic plasticity in the brain. Consistent with this hypothesis, spines contain high concentrations of actin, suggesting that they might be motile. To investigate this possibility, we made video recordings from hippocampal neurons expressing actin tagged with green fluorescent protein (GFP-actin). This reagent incorporates into actin-containing structures and allows the visualization of actin dynamics in living neurons. In mature neurons, recordings of GFP fluorescence revealed large actin-dependent changes in dendritic spine shape, similar to those inferred from previous studies using fixed tissues. Visible changes occurred within seconds, suggesting that anatomical plasticity at synapses can be extremely rapid. As well as providing a molecular basis for structural plasticity, the presence of motile actin in dendritic spines implicates the postsynaptic element as a primary site of this phenomenon.

Original languageEnglish (US)
Pages (from-to)847-854
Number of pages8
JournalNeuron
Volume20
Issue number5
DOIs
StatePublished - May 1998
Externally publishedYes

Fingerprint

Dendritic Spines
Actins
Neurons
Video Recording
Neuronal Plasticity
Green Fluorescent Proteins
Synapses
Spine
Fluorescence
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rapid actin-based plasticity in dendritic spines. / Fischer, Maria; Kaech-Petrie, Stefanie; Knutti, Darko; Matus, Andrew.

In: Neuron, Vol. 20, No. 5, 05.1998, p. 847-854.

Research output: Contribution to journalArticle

Fischer, Maria ; Kaech-Petrie, Stefanie ; Knutti, Darko ; Matus, Andrew. / Rapid actin-based plasticity in dendritic spines. In: Neuron. 1998 ; Vol. 20, No. 5. pp. 847-854.
@article{cff578e44fbc400393bb60b6b62c3ed9,
title = "Rapid actin-based plasticity in dendritic spines",
abstract = "Dendritic spines have been proposed as primary sites of synaptic plasticity in the brain. Consistent with this hypothesis, spines contain high concentrations of actin, suggesting that they might be motile. To investigate this possibility, we made video recordings from hippocampal neurons expressing actin tagged with green fluorescent protein (GFP-actin). This reagent incorporates into actin-containing structures and allows the visualization of actin dynamics in living neurons. In mature neurons, recordings of GFP fluorescence revealed large actin-dependent changes in dendritic spine shape, similar to those inferred from previous studies using fixed tissues. Visible changes occurred within seconds, suggesting that anatomical plasticity at synapses can be extremely rapid. As well as providing a molecular basis for structural plasticity, the presence of motile actin in dendritic spines implicates the postsynaptic element as a primary site of this phenomenon.",
author = "Maria Fischer and Stefanie Kaech-Petrie and Darko Knutti and Andrew Matus",
year = "1998",
month = "5",
doi = "10.1016/S0896-6273(00)80467-5",
language = "English (US)",
volume = "20",
pages = "847--854",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "5",

}

TY - JOUR

T1 - Rapid actin-based plasticity in dendritic spines

AU - Fischer, Maria

AU - Kaech-Petrie, Stefanie

AU - Knutti, Darko

AU - Matus, Andrew

PY - 1998/5

Y1 - 1998/5

N2 - Dendritic spines have been proposed as primary sites of synaptic plasticity in the brain. Consistent with this hypothesis, spines contain high concentrations of actin, suggesting that they might be motile. To investigate this possibility, we made video recordings from hippocampal neurons expressing actin tagged with green fluorescent protein (GFP-actin). This reagent incorporates into actin-containing structures and allows the visualization of actin dynamics in living neurons. In mature neurons, recordings of GFP fluorescence revealed large actin-dependent changes in dendritic spine shape, similar to those inferred from previous studies using fixed tissues. Visible changes occurred within seconds, suggesting that anatomical plasticity at synapses can be extremely rapid. As well as providing a molecular basis for structural plasticity, the presence of motile actin in dendritic spines implicates the postsynaptic element as a primary site of this phenomenon.

AB - Dendritic spines have been proposed as primary sites of synaptic plasticity in the brain. Consistent with this hypothesis, spines contain high concentrations of actin, suggesting that they might be motile. To investigate this possibility, we made video recordings from hippocampal neurons expressing actin tagged with green fluorescent protein (GFP-actin). This reagent incorporates into actin-containing structures and allows the visualization of actin dynamics in living neurons. In mature neurons, recordings of GFP fluorescence revealed large actin-dependent changes in dendritic spine shape, similar to those inferred from previous studies using fixed tissues. Visible changes occurred within seconds, suggesting that anatomical plasticity at synapses can be extremely rapid. As well as providing a molecular basis for structural plasticity, the presence of motile actin in dendritic spines implicates the postsynaptic element as a primary site of this phenomenon.

UR - http://www.scopus.com/inward/record.url?scp=0032078861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032078861&partnerID=8YFLogxK

U2 - 10.1016/S0896-6273(00)80467-5

DO - 10.1016/S0896-6273(00)80467-5

M3 - Article

C2 - 9620690

AN - SCOPUS:0032078861

VL - 20

SP - 847

EP - 854

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 5

ER -