RAP1 protein activates and silences transcription of mating-type genes in yeast

S. Kurtz, D. Shore

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

RAP1 is a sequence-specific DNA-binding protein essential for cell growth. The occurrence of RAP1-binding sites in many promoter regions, the mating-type gene silencer elements, and telomeres suggests that RAP1 has multiple functions in the cell. To assess its role in transcription, temperature-sensitive mutations in RAP1 were generated. Analysis of rap1(ts) strains provides evidence that RAP1 functions in both transcriptional activation and silencing of mating-type genes. Several observations indicate that rap1(ts) strains are defective in the expression of MATα, whose upstream activation sequence (UAS) contains a RAP1-binding site. At nonpermissive temperatures, decreases in MATα steady-state transcript levels can be detected in MATα rap1(ts) strains. Furthermore, these strains are deficient in α-pheromone production and simultaneously express at least two a-specific genes. These phenotypes can be reversed by replacing the RAP1-binding site at MATα with a binding site for the GAL4 transcriptional activator. Certain rap1(ts) alleles have an opposite effect on the silent mating-type locus HMR, which becomes partially derepressed at nonpermissive temperatures.

Original languageEnglish (US)
Pages (from-to)616-628
Number of pages13
JournalGenes and Development
Volume5
Issue number4
StatePublished - 1991
Externally publishedYes

Fingerprint

Yeasts
Binding Sites
Genes
Temperature
Proteins
Transcriptional Silencer Elements
Pheromones
Telomere
DNA-Binding Proteins
Gene Silencing
Genetic Promoter Regions
Transcriptional Activation
Alleles
Phenotype
Mutation
Growth

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

RAP1 protein activates and silences transcription of mating-type genes in yeast. / Kurtz, S.; Shore, D.

In: Genes and Development, Vol. 5, No. 4, 1991, p. 616-628.

Research output: Contribution to journalArticle

@article{86b98c67dddb41bbadf83b2ab8bca0e0,
title = "RAP1 protein activates and silences transcription of mating-type genes in yeast",
abstract = "RAP1 is a sequence-specific DNA-binding protein essential for cell growth. The occurrence of RAP1-binding sites in many promoter regions, the mating-type gene silencer elements, and telomeres suggests that RAP1 has multiple functions in the cell. To assess its role in transcription, temperature-sensitive mutations in RAP1 were generated. Analysis of rap1(ts) strains provides evidence that RAP1 functions in both transcriptional activation and silencing of mating-type genes. Several observations indicate that rap1(ts) strains are defective in the expression of MATα, whose upstream activation sequence (UAS) contains a RAP1-binding site. At nonpermissive temperatures, decreases in MATα steady-state transcript levels can be detected in MATα rap1(ts) strains. Furthermore, these strains are deficient in α-pheromone production and simultaneously express at least two a-specific genes. These phenotypes can be reversed by replacing the RAP1-binding site at MATα with a binding site for the GAL4 transcriptional activator. Certain rap1(ts) alleles have an opposite effect on the silent mating-type locus HMR, which becomes partially derepressed at nonpermissive temperatures.",
author = "S. Kurtz and D. Shore",
year = "1991",
language = "English (US)",
volume = "5",
pages = "616--628",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "4",

}

TY - JOUR

T1 - RAP1 protein activates and silences transcription of mating-type genes in yeast

AU - Kurtz, S.

AU - Shore, D.

PY - 1991

Y1 - 1991

N2 - RAP1 is a sequence-specific DNA-binding protein essential for cell growth. The occurrence of RAP1-binding sites in many promoter regions, the mating-type gene silencer elements, and telomeres suggests that RAP1 has multiple functions in the cell. To assess its role in transcription, temperature-sensitive mutations in RAP1 were generated. Analysis of rap1(ts) strains provides evidence that RAP1 functions in both transcriptional activation and silencing of mating-type genes. Several observations indicate that rap1(ts) strains are defective in the expression of MATα, whose upstream activation sequence (UAS) contains a RAP1-binding site. At nonpermissive temperatures, decreases in MATα steady-state transcript levels can be detected in MATα rap1(ts) strains. Furthermore, these strains are deficient in α-pheromone production and simultaneously express at least two a-specific genes. These phenotypes can be reversed by replacing the RAP1-binding site at MATα with a binding site for the GAL4 transcriptional activator. Certain rap1(ts) alleles have an opposite effect on the silent mating-type locus HMR, which becomes partially derepressed at nonpermissive temperatures.

AB - RAP1 is a sequence-specific DNA-binding protein essential for cell growth. The occurrence of RAP1-binding sites in many promoter regions, the mating-type gene silencer elements, and telomeres suggests that RAP1 has multiple functions in the cell. To assess its role in transcription, temperature-sensitive mutations in RAP1 were generated. Analysis of rap1(ts) strains provides evidence that RAP1 functions in both transcriptional activation and silencing of mating-type genes. Several observations indicate that rap1(ts) strains are defective in the expression of MATα, whose upstream activation sequence (UAS) contains a RAP1-binding site. At nonpermissive temperatures, decreases in MATα steady-state transcript levels can be detected in MATα rap1(ts) strains. Furthermore, these strains are deficient in α-pheromone production and simultaneously express at least two a-specific genes. These phenotypes can be reversed by replacing the RAP1-binding site at MATα with a binding site for the GAL4 transcriptional activator. Certain rap1(ts) alleles have an opposite effect on the silent mating-type locus HMR, which becomes partially derepressed at nonpermissive temperatures.

UR - http://www.scopus.com/inward/record.url?scp=0025809264&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025809264&partnerID=8YFLogxK

M3 - Article

C2 - 2010087

AN - SCOPUS:0025809264

VL - 5

SP - 616

EP - 628

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 4

ER -